Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes

Juan Manuel Falcón

Exosomal membrane proteins as interesting tool to express nonexosomal proteins into exosomes with therapeutic purposes.

Detection of amyloid fibrils in Parkinson’s disease using plasmonic chirality

Joaquín Castilla

This report shows the use of protein fibrils as templates for chiral nanoparticle assembly and development of a biodetection technique, to identify patients with Parkinson’s disease from human brain homogenates.

Metabolomic Identification of Subtypes of Nonalcoholic Steatohepatitis

José M Mato

We identified specific panels of markers that could distinguish patients with NASH from patients with simple steatosis for each subtype of NAFLD.

Sox2 promotes tamoxifen resistance in breast cancer cells

María Vivanco

Development of tamoxifen resistance is driven by Sox2‐dependent activation of Wnt signalling in cancer stem/progenitor cells.

Selected for Cover

Structural Mechanism for Cargo Recognition by the Retromer Complex 

Aitor Hierro

Defects in retromer impair various cellular processes and underlie some forms of Alzheimer’s disease and Parkinson’s disease.

MiRanalyzer: an update on the detection and analysis of microRNAs in high-throughput sequencing experiments

Ana M Aransay

Stand-alone version of the miRanalyzer that is based on a local and easily customized database is also available at https://bioinfo5.ugr.es/srnatoolbox/

MiRanalyzer: a microRNA detection and analysis tool for next-generation sequencing experiments

Ana M Aransay

The web tool provides a comprehensive overview of microRNA analysis.

Loss of the glycine N-methyltransferase gene leads to steatosis and hepatocellular carcinoma in mice

José M Mato

Methylation of RASSF1 and SOCS2 promoters and the binding of trimethylated lysine 27 in histone 3 to these 2 genes was increased in Hepatocellular Cacinomas from GNMT-KO mice

Obesity-Dependent Metabolic Signatures Associated with Nonalcoholic Fatty Liver Disease Progression

José M Mato

BMI-dependent serum metabolic profile may be able to reliably distinguish NASH from steatosis patients, have significant implications for the development of NASH biomarkers.

Liquid Chromatography-Mass Spectrometry-Based Parallel Metabolic Profiling of Human and Mouse Model Serum Reveals Putative Biomarkers Associated with the Progression of Nonalcoholic Fatty Liver Disease

José M Mato

Metabolic phenotyping approach may have a future role as a supplement for clinical decision making in NAFLD and in the adaption to more individualized treatment protocols.

Structure of ratcheted ribosomes with tRNAs in hybrid states

Mikel Valle

During protein synthesis, the displacement of the A-site tRNA on the 50S subunit is passive and is induced by the 30S subunit rotation, catalyzed by elongation factor G.

Wnt-11 promotes neuroendocrine-like differentiation, survival and migration of prostate cancer cells

Robert Kypta

Increased level of Wnt-11 found in prostate cancer contributes to tumour progression by promoting NED, tumour cell survival and cell migration/invasion.

Structural Basis for the Aminoacid Composition of Proteins from Halophilic Archea

Óscar Millet

We show that it is possible to decrease the salt dependence of a typical halophilic protein to the level of a mesophilic form and engineer a protein from a mesophilic organism into an obligate halophilic form.

Evolution of Complex RNA Polymerases: The Complete Archaeal RNA Polymerase Structure

Nicola Abrescia

The archaeal RNA polymerase (RNAP) shares structural similarities with eukaryotic RNAP II and we have determined the structure of the 13-subunit DNA-directed RNAP from Sulfolobus shibatae at 3.35 Å resolution.

Regulation of prostate cell growth and morphogenesis by Dickkopf-3

Robert Kypta

Loss of Dkk-3 expression resulting in impairment of glandular structure and uncontrolled prostate epithelial cell (PrEC) proliferation, both of which are crucial for prostate cancer progression.