Óscar Millet
omillet
Óscar Millet
PRINCIPAL INVESTIGATOR
Precision Medicine and Metabolism Lab
Orcid: 0000-0001-8748-4105
Email: omillet@cicbiogune.es
Phone: 4311 / 946 572 504
Address: Bizkaia Science and Technology Park, building 800, Derio (Bizkaia)
jmmato
José M Mato
General Director
General Director Office & Precision Medicine and Metabolism Lab
Orcid: 0000-0003-1264-3153
Email: director@cicbiogune.es
Phone: 4203 / 946 572 517
Address: Bizkaia Science and Technology Park, building 801A, Derio (Bizkaia)

He holds degrees in Chemistry (Univ. Ramon Llull,1994) and Chemical Engineering (IQS, 1995). After obtaining a Ph D in Organic Chemistry (University of Barcelona, 1999) he joined the group of Lewis Kay in Toronto for a post-doctoral stay (University of Toronto, 2000-2004). He was then recipient of a Ramon y Cajal reincorporation contract at the Parc Cientific de Barcelona (2004-2006) and he currently is group leader at the CIC bioGUNE. His research line focuses of the use of nuclear magnetic resonance (NMR) to the study of biologically relevant proteins and enzymes, paying special attention to the delicate balance existing between protein stability and dynamics. Such knowledge is applied for the development of new compounds with therapeutic activity, specifically in the field of rare diseases. He have published more than 55 papers with a total number of citations (1998-2015) of 2050 and an h-index of 17. He was the Spanish delegate for the trans domain of the COST program (2009-2014). He was awarded the prize of the Real Sociedad Española de Química (2004) and the Spanish NMR group prize (2005). He currently is the president of the Spanish NMR group.

Latest Publications
Metabolomics and lipidomics in NAFLD: biomarkers and non-invasive diagnostic tests
Masoodi, M;Gastaldelli, A;Hyotylainen, T;Arretxe, E;Alonso, C;Gaggini, M;Brosnan, J;Anstee, QM;Millet, O;Ortiz, P;Mato, JM;Dufour, JF;Oresic, M
NATURE REVIEWS GASTROENTEROLOGY & HEPATOLOGY
2021-09-10
O-GlcNAcylated p53 in the liver modulates hepatic glucose production
Gonzalez-Rellan, MJ;Fondevila, MF;Fernandez, U;Rodriguez, A;Varela-Rey, M;Veyrat-Durebex, C;Seoane, S;Bernardo, G;Lopitz-Otsoa, F;Fernandez-Ramos, D;Bilbao, J;Iglesias, C;Novoa, E;Ameneiro, ...
NATURE COMMUNICATIONS
2021-08-20
Integrative Modeling of Plasma Metabolic and Lipoprotein Biomarkers of SARS-CoV-2 Infection in Spanish and Australian COVID-19 Patient Cohorts
Masuda, R;Lodge, S;Nitschke, P;Spraul, M;Schaefer, H;Bong, SH;Kimhofer, T;Hall, D;Loo, RL;Bizkarguenaga, M;Bruzzone, C;Gil-Redondo, R;Embade, N;Mato, JM;Holmes, E;Wist, J;Millet, O;Nicholson, ...
JOURNAL OF PROTEOME RESEARCH
2021-08-06
A molecular signature for the metabolic syndrome by urine metabolomics
Bruzzone, C;Gil-Redondo, R;Seco, M;Barragan, R;de la Cruz, L;Cannet, C;Schafer, H;Fang, F;Diercks, T;Bizkarguenaga, M;Gonzalez-Valle, B;Lain, A;Sanz-Parra, A;Coltell, O;de Letona, AL;Spraul, ...
CARDIOVASCULAR DIABETOLOGY
2021-07-28
Diagnostic Potential of the Plasma Lipidome in Infectious Disease: Application to Acute SARS-CoV-2 Infection
Gray, N;Lawler, NG;Zeng, ANX;Ryan, M;Bong, SH;Boughton, BA;Bizkarguenaga, M;Bruzzone, C;Embade, N;Wist, J;Holmes, E;Millet, O;Nicholson, JK;Whiley, L
METABOLITES
2021-07-01
Metabolic Landscape of the Mouse Liver by Quantitative P-31 Nuclear Magnetic Resonance Analysis of the Phosphorome
Bernardo-Seisdedos, G;Bilbao, J;Fernandez-Ramos, D;Lopitz-Otsoa, F;de Juan, VG;Bizkarguenaga, M;Mateos, B;Fondevila, MF;Abril-Fornaguera, J;Diercks, T;Lu, SC;Nogueiras, R;Mato, JM;Millet, O
HEPATOLOGY
2021-06-15
Identification of novel UROS mutations in a patient with congenital erythropoietic porphyria and efficient treatment by phlebotomy
Blouin, JM;Ged, C;Bernardo-Seisdedos, G;Cabantous, T;Pinson, B;Poli, A;Puy, H;Millet, O;Gouya, L;Morice-Picard, F;Richard, E
MOLECULAR GENETICS AND METABOLISM REPORTS
2021-06-01
NMR of glycoproteins: profiling, structure, conformation and interactions
Unione, L;Arda, A;Jimenez-Barbero, J;Millet, O
CURRENT OPINION IN STRUCTURAL BIOLOGY
2021-06-01
Improving the Pharmacological Properties of Ciclopirox for Its Use in Congenital Erythropoietic Porphyria
Bernardo-Seisdedos, G;Charco, JM;SanJuan, I;Garcia-Martinez, S;Urquiza, P;Erana, H;Castilla, J;Millet, O
JOURNAL OF PERSONALIZED MEDICINE
2021-06-01
Cosolute modulation of protein oligomerization reactions in the homeostatic timescale
Mateos, B;Bernardo-Seisdedos, G;Dietrich, V;Zalba, N;Ortega, G;Peccati, F;Jimenez-Oses, G;Konrat, R;Tollinger, M;Millet, O
BIOPHYSICAL JOURNAL
2021-05-18
Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals
Egia-Mendikute, L;Bosch, A;Prieto-Fernandez, E;Lee, SY;Jimenez-Lasheras, B;del Rio, AG;Antonana-Vildosola, A;Bruzzone, C;Bizkarguenaga, M;Embade, N;Gil-Redondo, R;Martinez-Chantar, ML;Lopez-Hoyos, ...
COMMUNICATIONS BIOLOGY
2021-04-20
Therapeutic Targeting of Fumaryl Acetoacetate Hydrolase in Hereditary Tyrosinemia Type I
Gil-Martinez, J;Macias, I;Unione, L;Bernardo-Seisdedos, G;Lopitz-Otsoa, F;Fernandez-Ramos, D;Lain, A;Sanz-Parra, A;Mato, JM;Millet, O
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
2021-02-01

2020

Fluorinated Carbohydrates as Lectin Ligands: Simultaneous Screening of a Monosaccharide Library and Chemical Mapping by F-19 NMR Spectroscopy
Martinez, JD;Manzano, AI;Calvino, E;de Diego, A;de Francisco, BR;Romano, C;Oscarson, S;Millet, O;Gabius, HJ;Jimenez-Barbero, J;Canada, FJ
JOURNAL OF ORGANIC CHEMISTRY
2020-12-18
Unravelling the Time Scale of Conformational Plasticity and Allostery in Glycan Recognition by Human Galectin-1
Bertuzzi, S;Gimeno, A;Nunez-Franco, R;Bernardo-Seisdedos, G;Delgado, S;Jimenez-Oses, G;Millet, O;Jimenez-Barbero, J;Arda, A
CHEMISTRY-A EUROPEAN JOURNAL
2020-10-29
SARS-CoV-2 Infection Dysregulates the Metabolomic and Lipidomic Profiles of Serum
Bruzzone, C;Bizkarguenaga, M;Gil-Redondo, R;Diercks, T;Arana, E;de Vicuna, AG;Seco, M;Bosch, A;Palazon, A;San Juan, I;Lain, A;Gil-Martinez, J;Bernardo-Seisdedos, G;Fernandez-Ramos, D;Lopitz-Otsoa, ...
ISCIENCE
2020-10-23
Structural Characterization of N-Linked Glycans in the Receptor Binding Domain of the SARS-CoV-2 Spike Protein and their Interactions with Human Lectins
Lenza, MP;Oyenarte, I;Diercks, T;Quintana, JI;Gimeno, A;Coelho, H;Diniz, A;Peccati, F;Delgado, S;Bosch, A;Valle, M;Millet, O;Abrescia, NGA;Palazn, A;Marcelo, F;Jimnez-Oss, G;Jimnez-Barbero, ...
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
2020-10-22
Abnormal concentration of porphyrins in serum from COVID-19 patients
San Juan, I;Bruzzone, C;Bizkarguenaga, M;Bernardo-Seisdedos, G;Lain, A;Gil-Redondo, R;Diercks, T;Gil-Martinez, J;Urquiza, P;Arana, E;Seco, M;de Vicuna, AG;Embade, N;Mato, JM;Millet, O
BRITISH JOURNAL OF HAEMATOLOGY
2020-08-29
Scientific Response to the Coronavirus Crisis in Spain: Collaboration and Multidisciplinarity
Millet, O;Cortajarena, AL;Salvatella, X;Kiessling, LL;Jimenez-Barbero, J
ACS CHEMICAL BIOLOGY
2020-07-17
H-1 NMR-Based Urine Metabolomics Reveals Signs of Enhanced Carbon and Nitrogen Recycling in Prostate Cancer
Bruzzone, C;Loizaga-Iriarte, A;Sanchez-Mosquera, P;Gil-Redondo, R;Astobiza, I;Diercks, T;Cortazar, AR;Ugalde-Olano, A;Schafer, H;Blanco, FJ;Unda, M;Cannet, C;Spraul, M;Mato, JM;Embade, N;Carracedo, ...
JOURNAL OF PROTEOME RESEARCH
2020-06-05
An Epoxide Intermediate in Glycosidase Catalysis
Sobala, LF;Speciale, G;Zhu, S;Raich, L;Sannikova, N;Thompson, AJ;Hakki, Z;Lu, D;Abadi, SSK;Lewis, AR;Rojas-Cervellera, V;Bernardo-Seisdedos, G;Zhang, YM;Millet, O;Jimenez-Barbero, J;Bennet, ...
ACS CENTRAL SCIENCE
2020-05-27
Study of the Metabolomics of Equine Preovulatory Follicular Fluid: A Way to Improve Current In Vitro Maturation Media
Fernandez-Hernandez, P;Sanchez-Calabuig, MJ;Garcia-Marin, LJ;Bragado, MJ;Gutierrez-Adan, A;Millet, O;Bruzzone, C;Gonzalez-Fernandez, L;Macias-Garcia, B
ANIMALS
2020-05-01

2019

Modulation of the potassium channel KcsA by anionic phospholipids: Role of arginines at the non-annular lipid binding sites
Poveda, JA;Giudici, AM;Renart, ML;Millet, O;Morales, A;Gonzalez-Ros, JM;Oakes, V;Furini, S;Domene, C
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
2019-10-01
Glycoprofile Analysis of an Intact Glycoprotein As Inferred by NMR Spectroscopy
Unione, L;Lenza, MP;Arda, A;Urquiza, P;Lain, A;Falcon-Perez, JM;Jimenez-Barbero, J;Millet, O
ACS CENTRAL SCIENCE
2019-09-25
NMR-based newborn urine screening for optimized detection of inherited errors of metabolism
Embade, N;Cannet, C;Diercks, T;Gil-Redondo, R;Bruzzone, C;Anso, S;Echevarria, LR;Ayucar, MMM;Collazos, L;Lodoso, B;Guerra, E;Elorriaga, IA;Kortajarena, MA;Legorburu, AP;Fang, F;Astigarraga, ...
SCIENTIFIC REPORTS
2019-09-10
Hereditary tyrosinemia type I-associated mutations in fumarylacetoacetate hydrolase reduce the enzyme stability and increase its aggregation rate
Macias, I;Lain, A;Bernardo-Seisdedos, G;Gil, D;Gonzalez, E;Falcon-Perez, JM;Millet, O
JOURNAL OF BIOLOGICAL CHEMISTRY
2019-08-30
Side chain to main chain hydrogen bonds stabilize a polyglutamine helix in a transcription factor
Escobedo, A;Topal, B;Kunze, MBA;Aranda, J;Chiesa, G;Mungianu, D;Bernardo-Seisdedos, G;Eftekharzadeh, B;Gairi, M;Pierattelli, R;Felli, IC;Diercks, T;Millet, O;Garcia, J;Orozco, M;Crehuet, R;Lindorff-Larsen, ...
NATURE COMMUNICATIONS
2019-05-02

2018

Bioengineered PBGD variant improves the therapeutic index of gene therapy vectors for acute intermittent porphyria
Serrano-Mendioroz, I;Sampedro, A;Serna, N;de Salamanca, RE;Sanz-Parra, A;Corrales, F;Berraondo, P;Millet, O;Fontanellas, A
HUMAN MOLECULAR GENETICS
2018-11-01
Directional coupling of oligodendrocyte connexin-47 and astrocyte connexin-43 gap junctions
Fasciani, I;Pluta, P;Gonzalez-Nieto, D;Martinez-Montero, P;Molano, J;Paino, CL;Millet, O;Barrio, LC
GLIA
2018-11-01
Repurposing ciclopirox as a pharmacological chaperone in a model of congenital erythropoietic porphyria
Urquiza, P;Lain, A;Sanz-Parra, A;Moreno, J;Bernardo-Seisdedos, G;Dubus, P;Gonzalez, E;Gutierrez-de-Juan, V;Garcia, S;Erana, H;Juan, IS;Macias, I;Ben Bdira, F;Pluta, P;Ortega, G;Oyarzabal, J;Gonzalez-Muniz, ...
SCIENCE TRANSLATIONAL MEDICINE
2018-09-19
Structural basis of pyrrole polymerization in human porphobilinogen deaminase
Pluta, P;Roversi, P;Bernardo-Seisdedos, G;Rojas, AL;Cooper, JB;Gu, S;Pickersgill, RW;Millet, O
BIOCHIMICA ET BIOPHYSICA ACTA
2018-09-01
Structural basis and energy landscape for the Ca2+ gating and calmodulation of the Kv7.2 K+ channel
Bernardo-Seisdedos, G;Nunez, E;Gomis, C;Malo, C;Villarroel, A;Millet, O
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
2018-03-06

2017

Disulfide driven folding for a conditionally disordered protein
Fraga, H;Pujols, J;Gil-Garcia, M;Roque, A;Bernardo-Seisdedos, G;Santambrogio, C;Bech-Serra, JJ;Canals, F;Bernado, P;Grandori, R;Millet, O;Ventura, S
SCIENTIFIC REPORTS
2017-12-05
In Vitro Approach To Identify Key Amino Acids in Low Susceptibility of Rabbit Prion Protein to Misfolding
Erana, H;Fernandez-Borges, N;Elezgarai, SR;Harrathi, C;Charco, JM;Chianini, F;Dagleish, MP;Ortega, G;Millet, O;Castilla, J
JOURNAL OF VIROLOGY
2017-12-01
A Metabolomics Signature Linked To Liver Fibrosis In The Serum Of Transplanted Hepatitis C Patients
Cano, A;Marino, Z;Millet, O;Martinez-Arranz, I;Navasa, M;Falcon-Perez, JM;Perez-Cormenzana, M;Caballeria, J;Embade, N;Forns, X;Bosch, J;Castro, A;Mato, JM
SCIENTIFIC REPORTS
2017-09-05
Missense UROS mutations causing congenital erythropoietic porphyria reduce UROS homeostasis that can be rescued by proteasome inhibition
Blouin, JM;Bernardo-Seisdedos, G;Sasso, E;Esteve, J;Ged, C;Lalanne, M;Sanz-Parra, A;Urquiza, P;de Verneuil, H;Millet, O;Richard, E
HUMAN MOLECULAR GENETICS
2017-04-15
Contribution of Shape and Charge to the Inhibition of a Family GH99 endo-alpha-1,2-Mannanase
Petricevic, M;Sobala, LF;Fernandes, PZ;Raich, L;Thompson, AJ;Bernardo-Seisdedos, G;Millet, O;Zhu, S;Sollogoub, M;Jimenez-Barbero, J;Rovira, C;Davies, GJ;Williams, SJ
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
2017-01-25
Molecular Determinants of Chronic Liver Disease as Studied by NMR-Metabolomics
Embade, N;Millet, O
CURRENT TOPICS IN MEDICINAL CHEMISTRY
2017-01-01

2016

Active-Site-Directed Inhibitors of Prolyl Oligopeptidase Abolish Its Conformational Dynamics
Lopez, A;Herranz-Trillo, F;Kotev, M;Gairi, M;Guallar, V;Bernado, P;Millet, O;Tarrago, T;Giralt, E
CHEMBIOCHEM : A EUROPEAN JOURNAL OF CHEMICAL BIOLOGY
2016-05-17
Metabolic Characterization of Advanced Liver Fibrosis in HCV Patients as Studied by Serum H-1-NMR Spectroscopy
Embade, N;Marino, Z;Diercks, T;Cano, A;Lens, S;Cabrera, D;Navasa, M;Falcon-Perez, JM;Caballeria, J;Castro, A;Bosch, J;Mato, JM;Millet, O
PLOS ONE
2016-05-09

2015

Halophilic Protein Adaptation Results from Synergistic Residue-Ion Interactions in the Folded and Unfolded States
Ortega, G;Diercks, T;Millet, O
CHEMISTRY & BIOLOGY
2015-12-17
A Three-protein Charge Zipper Stabilizes a Complex Modulating Bacterial Gene Silencing
Cordeiro, TN;Garcia, J;Bernado, P;Millet, O;Pons, M
THE JOURNAL OF BIOLOGICAL CHEMISTRY
2015-08-28

2014

Tuning intracellular homeostasis of human uroporphyrinogen III synthase by enzyme engineering at a single hotspot of congenital erythropoietic porphyria
ben Bdira, F;Gonzalez, E;Pluta, P;Lain, A;Sanz-Parra, A;Falcon-Perez, JM;Millet, O
HUMAN MOLECULAR GENETICS
2014-11-01
De Novo Designed Library of Linear Helical Peptides: An Exploratory Tool in the Discovery of Protein-Protein Interaction Modulators
Bonache, MA;Balsera, B;Lopez-Mendez, B;Millet, O;Brancaccio, D;Gomez-Monterrey, I;Carotenuto, A;Pavone, LM;Reille-Seroussi, M;Gagey-Eilstein, N;Vidal, M;de la Torre-Martinez, R;Fernandez-Carvajal, ...
ACS COMBINATORIAL SCIENCE
2014-05-01
The Mitochondrial Intermembrane Space Oxireductase Mia40 Funnels the Oxidative Folding Pathway of the Cytochrome c Oxidase Assembly Protein Cox19*
Fraga, H;Bech-Serra, JJ;Canals, F;Ortega, G;Millet, O;Ventura, S
JOURNAL OF BIOLOGICAL CHEMISTRY
2014-04-04
LDL receptor/lipoprotein recognition: endosomal weakening of ApoB and ApoE binding to the convex face of the LR5 repeat
Martinez-Olivan, J;Arias-Moreno, X;Velazquez-Campoy, A;Millet, O;Sancho, J
FEBS JOURNAL
2014-03-01
Pivoting between Calmodulin Lobes Triggered by Calcium in the Kv7.2/Calmodulin Complex
Alaimo, A;Alberdi, A;Gomis-Perez, C;Fernandez-Orth, J;Bernardo-Seisdedos, G;Malo, C;Millet, O;Areso, P;Villarroel, A
PLOS ONE
2014-01-28
Clicked bis-PEG-peptide conjugates for studying calmodulin-Kv7.2 channel binding
Bonache, MA;Alaimo, A;Malo, C;Millet, O;Villarroel, A;Gonzalez-Muniz, R
ORGANIC & BIOMOLECULAR CHEMISTRY
2014-01-01

2013

Therapeutic potential of proteasome inhibitors in congenital erythropoietic porphyria
Blouin, JM;Duchartre, Y;Costet, P;Lalanne, M;Ged, C;Lain, A;Millet, O;de Verneuil, H;Richard, E
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
2013-11-05
Transmembrane and Juxtamembrane Structure of alpha L Integrin in Bicelles
Surya, W;Li, Y;Millet, O;Diercks, T;Torres, J
PLOS ONE
2013-09-12
Protein Functional Dynamics in Multiple Timescales as Studied by NMR Spectroscopy
Ortega, G;Pons, M;Millet, O
DYNAMICS OF PROTEINS AND NUCLEIC ACIDS
2013-01-01

2012

Carbohydrate Affinity for the Glucose-Galactose Binding Protein Is Regulated by Allosteric Domain Motions
Ortega, G;Castano, D;Diercks, T;Millet, O
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
2012-12-05
Structural and functional analyses of the interaction of archaeal RNA polymerase with DNA
Wojtas, MN;Mogni, M;Millet, O;Bell, SD;Abrescia, NGA
NUCLEIC ACIDS RESEARCH
2012-10-01
Mutation of Ser-50 and Cys-66 in Snapin Modulates Protein Structure and Stability
Navarro, A;Encinar, JA;Lopez-Mendez, B;Aguado-Llera, D;Prieto, J;Gomez, J;Matinez-Cruz, LA;Millet, O;Gonzalez-Ros, JM;Fernandez-Ballester, G;Neira, JL;Ferrer-Montiel, A
BIOCHEMISTRY
2012-04-24
Hydration Dynamics of a Halophilic Protein in Folded and Unfolded States
Qvist, J;Ortega, G;Tadeo, X;Millet, O;Halle, B
JOURNAL OF PHYSICAL CHEMISTRY B
2012-03-15

2011

N-15 Relaxation NMR Studies of Prolyl Oligopeptidase, an 80 kDa Enzyme, Reveal a Pre-existing Equilibrium between Different Conformational States
Kichik, N;Tarrago, T;Claasen, B;Gairi, M;Millet, O;Giralt, E
CHEMBIOCHEM
2011-12-16
Halophilic enzyme activation induced by salts
Ortega, G;Lain, A;Tadeo, X;Lopez-Mendez, B;Castano, D;Millet, O
SCIENTIFIC REPORTS
2011-06-14
Macromolecular Crowding Fails To Fold a Globular Protein in Cells
Schlesinger, AP;Wang, YQ;Tadeo, X;Millet, O;Pielak, G
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
2011-06-01
Intracellular Rescue of the Uroporphyrinogen III Synthase Activity in Enzymes Carrying the Hotspot Mutation C73R
Fortian, A;Gonzalez, E;Castano, D;Falcon-Perez, JM;Millet, O
JOURNAL OF BIOLOGICAL CHEMISTRY
2011-04-15
Backbone chemical shifts assignments of d-allose binding protein in the free form and in complex with d-allose
Castano, D;Millet, O
BIOMOLECULAR NMR ASSIGNMENTS
2011-04-01
STRUCTURAL, THERMODYNAMIC, AND MECHANISTICAL STUDIES IN UROPORPHYRINOGEN III SYNTHASE: MOLECULAR BASIS OF CONGENITAL ERYTHROPOIETIC PORPHYRIA
Fortian, A;Castano, D;Gonzalez, E;Lain, A;Falcon-Perez, JM;Millet, O
ADVANCES IN PROTEIN CHEMISTRY AND STRUCTURAL BIOLOGY: PROTEIN STRUCTURE AND DISEASES, VOL 83
2011-01-01

2010

Thermodynamics of protein-cation interaction: Ca+2 and Mg+2 binding to the fifth binding module of the LDL receptor
Arias-Moreno, X;Cuesta-Lopez, S;Millet, O;Sancho, J;Velazquez-Campoy, A
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
2010-03-01

2009

Structural Basis for the Aminoacid Composition of Proteins from Halophilic Archea
Tadeo, X;Lopez-Mendez, B;Trigueros, T;Lain, A;Castano, D;Millet, O
PLOS BIOLOGY
2009-12-01
Protein Stabilization and the Hofmeister Effect: The Role of Hydrophobic Solvation
Tadeo, X;Lopez-Mendez, B;Castano, D;Trigueros, T;Millet, O
BIOPHYSICAL JOURNAL
2009-11-04
Uroporphyrinogen III Synthase Mutations Related to Congenital Erythropoietic Porphyria Identify a Key Helix for Protein Stability
Fortian, A;Castano, D;Ortega, G;Lain, A;Pons, M;Millet, O
BIOCHEMISTRY
2009-01-20

2007

Anion modulation of the H-1/H-2 exchange rates in backbone amide protons monitored by NMR spectroscopy
Tadeo, X;Castano, D;Millet, O
PROTEIN SCIENCE
2007-12-01
Influence of the hofmeister anions on protein stability as studied by thermal denaturation and chemical shift perturbation
Tadeo, X;Pons, M;Millet, O
BIOCHEMISTRY
2007-01-23

Protein stability (thermodynamic and kinetic) drives the biophysical properties of the polypeptide chain (protein folding) and the protein's concentration in the cellular environment (protein homeostasis). It is the result of a delicate balance between inter- and intramolecular interactions, which can be easily altered by mutations and/or upon changes in the composition of the surrounding media. In this context, NMR spectroscopy offers a plethora of suitable experiments to investigate protein stability. In our laboratory we are currently interested in the following topics:

  • Pharmacological chaperones. Rare diseases (~7000 identified to date) are an area of significant medical need affecting an estimated 350 million people worldwide, with ~95% having no currently approved drug treatment. They are often produced by inherited mutations affecting the activity of a protein and It is becoming increasily clear that, most frequently, a mutation destabilizes the protein/enzyme, ultimately affecting its intracellular homeostasis. In this context, pharmacological chaperones (small molecules which bind to the protein, restoring stability and activity without affecting its function) can be applied to many diseases. In our laboratory we are investigating new methods (NMR, biophysical and biochemical) for the discovery and characterization of pharmacological chaperones against a set of diseases: congenital eryhtropoietic porphyria, tyrosianemia.
     
  • Environmental modulation of enzyme stability. The high catalytic efficiency and the exquisite enantioselectivity of an enzyme has been employed in some industrial processes to upgrade their properties in order to make them more environmentally-friendly. However, large scale industrial implementation of biotechnological reactions is often limited by the marginal stability of the enzyme in the reactor conditions. In our laboratory we employ NMR and circular dichroism to investigate the effect of external crowding agents to improve the activity and stability of several enzymes. Specifically, we are investigating the mechanism for protein haloadaptation by a combined use of site directed mutagenesis and high-resolution NMR spectroscopy.

Protocolos de Histología

Latest Publications

Metabolomics and lipidomics in NAFLD: biomarkers and non-invasive diagnostic tests

Masoodi, M;Gastaldelli, A;Hyotylainen, T;Arretxe, E;Alonso, C;Gaggini, M;Brosnan, J;Anstee, QM;Millet, O;Ortiz, P;Mato, JM;Dufour, JF;Oresic, M

NATURE REVIEWS GASTROENTEROLOGY & HEPATOLOGY

2021-09-10

O-GlcNAcylated p53 in the liver modulates hepatic glucose production

Gonzalez-Rellan, MJ;Fondevila, MF;Fernandez, U;Rodriguez, A;Varela-Rey, M;Veyrat-Durebex, C;Seoane, S;Bernardo, G;Lopitz-Otsoa, F;Fernandez-Ramos, D;Bilbao, J;Iglesias, C;Novoa, E;Ameneiro, ...

NATURE COMMUNICATIONS

2021-08-20

Integrative Modeling of Plasma Metabolic and Lipoprotein Biomarkers of SARS-CoV-2 Infection in Spanish and Australian COVID-19 Patient Cohorts

Masuda, R;Lodge, S;Nitschke, P;Spraul, M;Schaefer, H;Bong, SH;Kimhofer, T;Hall, D;Loo, RL;Bizkarguenaga, M;Bruzzone, C;Gil-Redondo, R;Embade, N;Mato, JM;Holmes, E;Wist, J;Millet, O;Nicholson, ...

JOURNAL OF PROTEOME RESEARCH

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