Óscar Millet
omillet
Óscar Millet
PRINCIPAL INVESTIGATOR
Precision Medicine and Metabolism Lab
Orcid: 0000-0001-8748-4105
Email: omillet@cicbiogune.es
Phone: 4311 / 946 572 504
Address: Bizkaia Science and Technology Park, building 800, Derio (Bizkaia)

He holds degrees in Chemistry (Univ. Ramon Llull,1994) and Chemical Engineering (IQS, 1995). After obtaining a Ph D in Organic Chemistry (University of Barcelona, 1999) he joined the group of Lewis Kay in Toronto for a post-doctoral stay (University of Toronto, 2000-2004). He was then recipient of a Ramon y Cajal reincorporation contract at the Parc Cientific de Barcelona (2004-2006) and he currently is group leader at the CIC bioGUNE. His research line focuses of the use of nuclear magnetic resonance (NMR) to the study of biologically relevant proteins and enzymes, paying special attention to the delicate balance existing between protein stability and dynamics. Such knowledge is applied for the development of new compounds with therapeutic activity, specifically in the field of rare diseases. He have published more than 55 papers with a total number of citations (1998-2015) of 2050 and an h-index of 17. He was the Spanish delegate for the trans domain of the COST program (2009-2014). He was awarded the prize of the Real Sociedad Española de Química (2004) and the Spanish NMR group prize (2005). He currently is the president of the Spanish NMR group.

Latest Publications
Structural Insights into the Intracellular Region of the Human Magnesium Transport Mediator CNNM4
Gimenez-Mascarell, P (Gimenez-Mascarell, Paula); Oyenarte, I (Oyenarte, Iker); Gonzalez-Recio, I (Gonzalez-Recio, Irene); Fernandez-Rodriguez, C (Fernandez-Rodriguez, Carmen); Corral-Rodriguez, ...
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
2019-12-01
Ubiquitin-Like Post-Translational Modifications (Ubl-PTMs): Small Peptides with Huge Impact in Liver Fibrosis
Lachiondo-Ortega, S (Lachiondo-Ortega, Sofia); Mercado-Gomez, M (Mercado-Gomez, Maria); Serrano-Macia, M (Serrano-Macia, Marina); Lopitz-Otsoa, F (Lopitz-Otsoa, Fernando); Salas-Villalobos, ...
CELLS
2019-12-01
SerpinB3 Differently Up-Regulates Hypoxia Inducible Factors-1 alpha and-2 alpha in Hepatocellular Carcinoma: Mechanisms Revealing Novel Potential Therapeutic Targets
Cannito, S (Cannito, Stefania)[ 1 ] ; Foglia, B (Foglia, Beatrice); Villano, G (Villano, Gianmarco); Turato, C (Turato, Cristian); Delgado, TC (Delgado, Teresa C.); Morello, E (Morello, Elisabetta); ...
CANCERS
2019-12-01
Modulation of the potassium channel KcsA by anionic phospholipids: Role of arginines at the non-annular lipid binding sites
Poveda, JA;Giudici, AM;Renart, ML;Millet, O;Morales, A;Gonzalez-Ros, JM;Oakes, V;Furini, S;Domene, C
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
2019-10-01
Glycoprofile Analysis of an Intact Glycoprotein As Inferred by NMR Spectroscopy
Unione, L;Lenza, MP;Arda, A;Urquiza, P;Lain, A;Falcon-Perez, JM;Jimenez-Barbero, J;Millet, O
ACS CENTRAL SCIENCE
2019-09-25
NMR-based newborn urine screening for optimized detection of inherited errors of metabolism
Embade, N;Cannet, C;Diercks, T;Gil-Redondo, R;Bruzzone, C;Anso, S;Echevarria, LR;Ayucar, MMM;Collazos, L;Lodoso, B;Guerra, E;Elorriaga, IA;Kortajarena, MA;Legorburu, AP;Fang, F;Astigarraga, ...
SCIENTIFIC REPORTS
2019-09-10
Hereditary tyrosinemia type I-associated mutations in fumarylacetoacetate hydrolase reduce the enzyme stability and increase its aggregation rate
Macias, I;Lain, A;Bernardo-Seisdedos, G;Gil, D;Gonzalez, E;Falcon-Perez, JM;Millet, O
JOURNAL OF BIOLOGICAL CHEMISTRY
2019-08-30
Side chain to main chain hydrogen bonds stabilize a polyglutamine helix in a transcription factor
Escobedo, A;Topal, B;Kunze, MBA;Aranda, J;Chiesa, G;Mungianu, D;Bernardo-Seisdedos, G;Eftekharzadeh, B;Gairi, M;Pierattelli, R;Felli, IC;Diercks, T;Millet, O;Garcia, J;Orozco, M;Crehuet, R;Lindorff-Larsen, ...
NATURE COMMUNICATIONS
2019-05-02
Fine tuning of SIRT1 expression is essential to protect the liver from cholestasis
Blokker, B (Blokker, B.); Maijo, M (Maijo, M.); Philo, M (Philo, M.); Gutierrez, V (Gutierrez, V.); Halilbasic, E (Halilbasic, E.); Fuchs, C (Fuchs, C.); Legall, G (Legall, G.); Milkiewicz, ...
JOURNAL OF HEPATOLOGY
2019-04-01

2018

From 1,4-Disaccharide to 1,3-Glycosyl Carbasugar: Synthesis of a Bespoke Inhibitor of Family GH99 Endo-alpha-mannosidase
Lu, D(Lu, Dan);Zhu, S(Zhu, Sha);Sobala, LF(Sobala, Lukasz F.);Bernardo-Seisdedos, G(Bernardo-Seisdedos, Ganeko);Milet, O(Milet, Oscar);Zhang, YM(Zhang, Yongmin);Jimenez-Barbero, J(Jimenez-Barbero, ...
ORGANIC LETTERS
2018-12-07
Bioengineered PBGD variant improves the therapeutic index of gene therapy vectors for acute intermittent porphyria
Serrano-Mendioroz, I(Serrano-Mendioroz, Irantzu);Sampedro, A(Sampedro, Ana);Serna, N(Serna, Naroa);de Salamanca, RE(Enriquez de Salamanca, Rafael);Sanz-Parra, A(Sanz-Parra, Arantza);Corrales, ...
HUMAN MOLECULAR GENETICS
2018-11-01
Directional coupling of oligodendrocyte connexin-47 and astrocyte connexin-43 gap junctions
Fasciani, I(Fasciani, Ilaria);Pluta, P(Pluta, Paula);Gonzalez-Nieto, D(Gonzalez-Nieto, Daniel);Martinez-Montero, P(Martinez-Montero, Paloma);Molano, J(Molano, Jesus);Paino, CL(Paino, Carlos ...
GLIA
2018-11-01
Repurposing ciclopirox as a pharmacological chaperone in a model of congenital erythropoietic porphyria
Urquiza, P(Urquiza, Pedro);Lain, A(Lain, Ana);Sanz-Parra, A(Sanz-Parra, Arantza);Moreno, J(Moreno, Jorge);Bernardo-Seisdedos, G(Bernardo-Seisdedos, Ganeko);Dubus, P(Dubus, Pierre);Gonzalez, ...
SCIENCE TRANSLATIONAL MEDICINE
2018-09-19
Structural basis of pyrrole polymerization in human porphobilinogen deaminase
Pluta, P(Pluta, Paula);Roversi, P(Roversi, Pietro);Bernardo-Seisdedos, G(Bernardo-Seisdedos, Ganeko);Rojas, AL(Rojas, Adriana L.);Cooper, JB(Cooper, Jonathan B.);Gu, S(Gu, Shuang);Pickersgill, ...
BIOCHIMICA ET BIOPHYSICA ACTA
2018-09-01
Metabolomic-Based Noninvasive Serum Test to Diagnose Nonalcoholic Steatohepatitis: Results From Discovery and Validation Cohorts
Mayo, R(Mayo, Rebeca);Crespo, J(Crespo, Javier);Martinez-Arranz, I(Martinez-Arranz, Ibon);Banales, JM(Banales, Jesus M.);Arias, M(Arias, Mayte);Minchole, I(Minchole, Itziar);de la Fuente, RA(Aller ...
HEPATOLOGY COMMUNICATIONS
2018-07-01
Structural basis and energy landscape for the Ca2+ gating and calmodulation of the Kv7.2 K+ channel
Bernardo-Seisdedos, G(Bernardo-Seisdedos, Ganeko);Nunez, E(Nunez, Eider);Gomis, C(Gomis, Carolina);Malo, C(Malo, Covadonga);Villarroel, A(Villarroel, Alvaro);Millet, O(Millet, Oscar)
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
2018-03-06

2017

The mitochondrial negative regulator MCJ is a therapeutic target for acetaminophen-induced liver injury
Barbier-Torres, L(Barbier-Torres, Lucia);Iruzubieta, P(Iruzubieta, Paula);Fernandez-Ramos, D(Fernandez-Ramos, David);Delgado, TC(Delgado, Teresa C.);Taibo, D(Taibo, Daniel);Guitierrez-de-Juan, ...
NATURE COMMUNICATIONS
2017-12-12
Disulfide driven folding for a conditionally disordered protein
Fraga, H(Fraga, Hugo);Pujols, J(Pujols, Jordi);Gil-Garcia, M(Gil-Garcia, Marcos);Roque, A(Roque, Alicia);Bernardo-Seisdedos, G(Bernardo-Seisdedos, Ganeko);Santambrogio, C(Santambrogio, Carlo);Bech-Serra, ...
SCIENTIFIC REPORTS
2017-12-05
In Vitro Approach To Identify Key Amino Acids in Low Susceptibility of Rabbit Prion Protein to Misfolding
Erana, H(Erana, Hasier);Fernandez-Borges, N(Fernandez-Borges, Natalia);Elezgarai, SR(Elezgarai, Saioa R.);Harrathi, C(Harrathi, Chafik);Charco, JM(Charco, Jorge M.);Chianini, F(Chianini, Francesca);Dagleish, ...
JOURNAL OF VIROLOGY
2017-12-01
Role of Aramchol in Steatohepatitis and Fibrosis in Mice
Iruarrizaga-Lejarreta, M(Iruarrizaga-Lejarreta, Marta);Varela-Rey, M(Varela-Rey, Marta);Fernandez-Ramos, D(Fernandez-Ramos, David);Martinez-Arranz, I(Martinez-Arranz, Ibon);Delgado, TC(Delgado, ...
HEPATOLOGY COMMUNICATIONS
2017-11-01
The immunosuppressive effect of the tick protein, Salp15, is long-lasting and persists in a murine model of hematopoietic transplant
Tomas-Cortazar, J(Tomas-Cortazar, Julen);Martin-Ruiz, I(Martin-Ruiz, Itziar);Barriales, D(Barriales, Diego);Pascual-Itoiz, MA(Angel Pascual-Itoiz, Miguel);de Juan, VG(Gutierrez de Juan, Virginia);Caro-Maldonado, ...
SCIENTIFIC REPORTS
2017-09-06
A Metabolomics Signature Linked To Liver Fibrosis In The Serum Of Transplanted Hepatitis C Patients
Cano, A(Cano, Ainara);Marino, Z(Marino, Zoe);Millet, O(Millet, Oscar);Martinez-Arranz, I(Martinez-Arranz, Ibon);Navasa, M(Navasa, Miquel);Falcon-Perez, JM(Manuel Falcon-Perez, Juan);Perez-Cormenzana, ...
SCIENTIFIC REPORTS
2017-09-05
Metabolomic Identification of Subtypes of Nonalcoholic Steatohepatitis
Alonso, C(Alonso, Cristina);Fernandez-Ramos, D(Fernandez-Ramos, David);Varela-Rey, M(Varela-Rey, Marta);Martinez-Arranz, I(Martinez-Arranz, Ibon);Navasa, N(Navasa, Nicolas);Van Liempd, SM(Van ...
GASTROENTEROLOGY
2017-05-01
Missense UROS mutations causing congenital erythropoietic porphyria reduce UROS homeostasis that can be rescued by proteasome inhibition
Blouin, JM(Blouin, Jean-Marc);Bernardo-Seisdedos, G(Bernardo-Seisdedos, Ganeko);Sasso, E(Sasso, Emma);Esteve, J(Esteve, Julie);Ged, C(Ged, Cecile);Lalanne, M(Lalanne, Magalie);Sanz-Parra, A(Sanz-Parra, ...
HUMAN MOLECULAR GENETICS
2017-04-15
Contribution of Shape and Charge to the Inhibition of a Family GH99 endo-alpha-1,2-Mannanase
Petricevic, M(Petricevic, Marija);Sobala, LF(Sobala, Lukasz F.);Fernandes, PZ(Fernandes, Pearl Z.);Raich, L(Raich, Lluis);Thompson, AJ(Thompson, Andrew J.);Bernardo-Seisdedos, G(Bernardo-Seisdedos, ...
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
2017-01-25
Structural Basis of the Oncogenic Interaction of Phosphatase PRL-1 with the Magnesium Transporter CNNM2
Gimenez-Mascarell, P(Gimenez-Mascarell, Paula);Oyenarte, I(Oyenarte, Iker);Hardy, S(Hardy, Serge);Breiderhoff, T(Breiderhoff, Tilman);Stuiver, M(Stuiver, Marchel);Kostantin, E(Kostantin, Elie);Diercks, ...
THE JOURNAL OF BIOLOGICAL CHEMISTRY
2017-01-20
Molecular Determinants of Chronic Liver Disease as Studied by NMR-Metabolomics
Embade, N(Embade, Nieves);Millet, O(Millet, Oscar)
CURRENT TOPICS IN MEDICINAL CHEMISTRY
2017-01-01

2016

Unraveling the Conformational Landscape of Ligand Binding to Glucose/Galactose-Binding Protein by Paramagnetic NMR and MD Simulations
Unione, L(Unione, Luca);Ortega, G(Ortega, Gabriel);Mallaaray, A(Mallaaray, Alvaro);Corzana, F(Corzana, Francisco);Perez-Castells, J(Perez-Castells, Javier);Canales, A(Canales, Angeles);Jimenez-Barbero, ...
ACS CHEMICAL BIOLOGY
2016-08-01
Metabolomics as a diagnostic tool for idiopathic non-cirrhotic portal hypertension
Seijo, S(Seijo, Susana);Lozano, JJ(Jose Lozano, Juan);Alonso, C(Alonso, Cristina);Miquel, R(Miquel, Rosa);Berzigotti, A(Berzigotti, Annalisa);Reverter, E(Reverter, Enric);Turon, F(Turon, Fanny);Martinez-Chantar, ...
LIVER INTERNATIONAL : OFFICIAL JOURNAL OF THE INTERNATIONAL ASSOCIATION FOR THE STUDY OF THE LIVER
2016-07-01
Active-Site-Directed Inhibitors of Prolyl Oligopeptidase Abolish Its Conformational Dynamics
Lopez, A(Lopez, Abraham);Herranz-Trillo, F(Herranz-Trillo, Fatima);Kotev, M(Kotev, Martin);Gairi, M(Gairi, Margarida);Guallar, V(Guallar, Victor);Bernado, P(Bernado, Pau);Millet, O(Millet, Oscar);Tarrago, ...
CHEMBIOCHEM : A EUROPEAN JOURNAL OF CHEMICAL BIOLOGY
2016-05-17
Metabolic Characterization of Advanced Liver Fibrosis in HCV Patients as Studied by Serum H-1-NMR Spectroscopy
Embade, N(Embade, Nieves);Marino, Z(Marino, Zoe);Diercks, T(Diercks, Tammo);Cano, A(Cano, Ainara);Lens, S(Lens, Sabela);Cabrera, D(Cabrera, Diana);Navasa, M(Navasa, Miquel);Falcon-Perez, JM(Falcon-Perez, ...
PLOS ONE
2016-05-09
Neoangiogenesis-related genes are hallmarks of fast-growing hepatocellular carcinomas and worst survival. Results from a prospective study
Villa, E(Villa, Erica);Critelli, R(Critelli, Rosina);Lei, B(Lei, Barbara);Marzocchi, G(Marzocchi, Guido);Camma, C(Camma, Calogero);Giannelli, G(Giannelli, Gianluigi);Pontisso, P(Pontisso, Patrizia);Cabibbo, ...
GUT
2016-05-01
Methionine and S-adenosylmethionine levels are critical regulators of PP2A activity modulating lipophagy during steatosis
Zubiete-Franco, I(Zubiete-Franco, Imanol);Garcia-Rodriguez, JL(Garcia-Rodriguez, Juan Luis);Martinez-Una, M(Martinez-Una, Maite);Martinez-Lopez, N(Martinez-Lopez, Nuria);Woodhoo, A(Woodhoo, ...
JOURNAL OF HEPATOLOGY
2016-02-01

2015

Halophilic Protein Adaptation Results from Synergistic Residue-Ion Interactions in the Folded and Unfolded States
Ortega, G(Ortega, Gabriel);Diercks, T(Diercks, Tammo);Millet, O(Millet, Oscar)
CHEMISTRY & BIOLOGY
2015-12-17
A Three-protein Charge Zipper Stabilizes a Complex Modulating Bacterial Gene Silencing
Cordeiro, TN(Cordeiro, Tiago N.);Garcia, J(Garcia, Jesus);Bernado, P(Bernado, Pau);Millet, O(Millet, Oscar);Pons, M(Pons, Miquel)
THE JOURNAL OF BIOLOGICAL CHEMISTRY
2015-08-28
Repression of the Nuclear Receptor Small Heterodimer Partner by Steatotic Drugs and in Advanced Nonalcoholic Fatty Liver Disease
Benet, M(Benet, Marta);Guzman, C(Guzman, Carla);Pisonero-Vaquero, S(Pisonero-Vaquero, Sandra);Garcia-Mediavilla, MV(Victoria Garcia-Mediavilla, M.);Sanchez-Campos, S(Sanchez-Campos, Sonia);Martinez-Chantar, ...
MOLECULAR PHARMACOLOGY
2015-04-01
Regulation of Oxidative Stress by Methylation-Controlled J Protein Controls Macrophage Responses to Inflammatory Insults
Navasa, N(Navasa, Nicolas);Martin, I(Martin, Itziar);Iglesias-Pedraz, JM(Iglesias-Pedraz, Juan Manuel);Beraza, N(Beraza, Naiara);Atondo, E(Atondo, Estibaliz);Izadi, H(Izadi, Hooman);Ayaz, F(Ayaz, ...
THE JOURNAL OF INFECTIOUS DISEASES
2015-01-01
The Need for Biomarkers in Diagnosis and Prognosis of Drug-Induced Liver Disease: Does Metabolomics Have Any Role?
...
BIOMED RESEARCH INTERNATIONAL
2015-01-01

2014

Tuning intracellular homeostasis of human uroporphyrinogen III synthase by enzyme engineering at a single hotspot of congenital erythropoietic porphyria.
ben Bdira, Fredj; Gonzalez, Esperanza; Pluta, Paula; Lain, Ana; Sanz-Parra, Arantza; Manuel Falcon-Perez, Juan; Millet, Oscar
HUMAN MOLECULAR GENETICS
2014-11-01
Systems biology for hepatologists.
Mato, Jose M.; Luz Martinez-Chantar, M.; Lu, Shelly C.
HEPATOLOGY
2014-08-01
SIRT1 controls liver regeneration by regulating bile acid metabolism through farnesoid X receptor and mammalian target of rapamycin signaling.
Garcia-Rodriguez, Juan L.; Barbier-Torres, Lucia; Fernandez-Alvarez, Sara; Gutierrez-de Juan, Virginia; Monte, Maria J.; Halilbasic, Emina; Herranz, Daniel; Alvarez, Luis; Aspichueta, Patricia; ...
HEPATOLOGY
2014-05-01
De Novo Designed Library of Linear Helical Peptides: An Exploratory Tool in the Discovery of Protein-Protein Interaction Modulators
Angeles Bonache, M.; Balsera, Beatriz; Lopez-Mendez, Blanca; Millet, Oscar; Brancaccio, Diego; Gomez-Monterrey, Isabel; Carotenuto, Alfonso; Pavone, Luigi M.; Reille-Seroussi, Marie; Gagey-Eilstein, ...
ACS COMBINATORIAL SCIENCE
2014-05-01
The Mitochondrial Intermembrane Space Oxireductase Mia40 Funnels the Oxidative Folding Pathway of the Cytochrome c Oxidase Assembly Protein Cox19*
Fraga, Hugo; Bech-Serra, Joan-Josep; Canals, Francesc; Ortega, Gabriel; Millet, Oscar; Ventura, Salvador
JOURNAL OF BIOLOGICAL CHEMISTRY
2014-04-04
LDL receptor/lipoprotein recognition: endosomal weakening of ApoB and ApoE binding to the convex face of the LR5 repeat
Martinez-Olivan, Juan; Arias-Moreno, Xabier; Velazquez-Campoy, Adrian; Millet, Oscar; Sancho, Javier
FEBS JOURNAL
2014-03-01
Pivoting between calmodulin lobes triggered by calcium in the Kv7.2/calmodulin complex.
Alaimo, Alessandro; Alberdi, Araitz; Gomis-Perez, Carolina; Fernandez-Orth, Juncal; Bernardo-Seisdedos, Ganeko; Malo, Covadonga; Millet, Oscar; Areso, Pilar; Villarroel, Alvaro
PLOS ONE
2014-01-01

-0001

Clicked bis-PEG-peptide conjugates for studying calmodulin-Kv7.2 channel binding
Angeles Bonache, M.; Alaimo, Alessandro; Malo, Covadonga; Millet, Oscar; Villarroel, Alvaro; Gonzalez-Muniz, Rosario
ORGANIC & BIOMOLECULAR CHEMISTRY
2014

2017

Protein Functional Dynamics in Multiple Timescales as Studied by NMR Spectroscopy
Ortega, Gabriel; Pons, Miquel; Millet, Oscar
DYNAMICS OF PROTEINS AND NUCLEIC ACIDS
2017-07-28

2013

Therapeutic potential of proteasome inhibitors in congenital erythropoietic porphyria
Blouin, Jean-Marc; Duchartre, Yann; Costet, Pierre; Lalanne, Magalie; Ged, Cecile; Lain, Ana; Millet, Oscar; de Verneuil, Hubert; Richard, Emmanuel
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
2013-11-05
Excess S-adenosylmethionine Reroutes Phosphatidylethanolamine Towards Phosphatidylcholine and Triglyceride Synthesis
Martinez-Una, Maite; Varela-Rey, Marta; Cano, Ainara; Fernandez-Ares, Larraitz; Beraza, Naiara; Aurrekoetxea, Igor; Martinez-Arranz, Ibon; Garcia-Rodriguez, Juan L.; Buque, Xabier; Mestre, Daniela; ...
HEPATOLOGY
2013-10-01
Transmembrane and Juxtamembrane Structure of alpha L Integrin in Bicelles
Surya, Wahyu; Li, Yan; Millet, Oscar; Diercks, Tammo; Torres, Jaume
PLOS ONE
2013-09-12
A DNA methylation signature associated with the epigenetic repression of glycine N-methyltransferase in human hepatocellular carcinoma
Huidobro, Covadonga; Torano, Estela G.; Fernandez, Agustin F.; Urdinguio, Rocio G.; Rodriguez, Ramon M.; Ferrero, Cecilia; Martinez-Camblor, Pablo; Boix, Loreto; Bruix, Jordi; Luis Garcia-Rodriguez, ...
JOURNAL OF MOLECULAR MEDICINE-JMM
2013-08-01
Metabolomics Discloses Potential Biomarkers for the Noninvasive Diagnosis of Idiopathic Portal Hypertension
Seijo, Susana; Jose Lozano, Juan; Alonso, Cristina; Reverter, Enric; Miquel, Rosa; Abraldes, Juan G.; Luz Martinez-Chantar, Maria; Garcia-Criado, Angeles; Berzigotti, Annalisa; Castro, Azucena; ...
AMERICAN JOURNAL OF GASTROENTEROLOGY
2013-06-01
The human liver fatty acid binding protein (FABP1) gene is activated by FOXA1 and PPAR alpha; and repressed by C/EBP alpha: Implications in FABP1 down-regulation in nonalcoholic fatty liver disease
Guzman, Carla; Benet, Marta; Pisonero-Vaquero, Sandra; Moya, Marta; Victoria Garcia-Mediavilla, M.; Luz Martinez-Chantar, M.; Gonzalez-Gallego, Javier; Vicente Castell, Jose; Sanchez-Campos, ...
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
2013-04-01

2012

Carbohydrate Affinity for the Glucose-Galactose Binding Protein Is Regulated by Allosteric Domain Motions
Ortega, Gabriel; Castano, David; Diercks, Tammo; Millet, Oscar
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
2012-12-05
Mitochondrial GSH determines the toxic or therapeutic potential of superoxide scavenging in steatohepatitis
von Montfort, Claudia; Matias, Nuria; Fernandez, Anna; Fucho, Raquel; Conde de la Rosa, Laura; Luz Martinez-Chantar, Maria; Mato, Jose M.; Machida, Keigo; Tsukamoto, Hidekazu; Murphy, Michael ...
JOURNAL OF HEPATOLOGY
2012-10-01
Structural and functional analyses of the interaction of archaeal RNA polymerase with DNA
Wojtas, Magdalena N.; Mogni, Maria; Millet, Oscar; Bell, Stephen D.; Abrescia, Nicola G. A.
NUCLEIC ACIDS RESEARCH
2012-10-01
Mutation of Ser-50 and Cys-66 in Snapin Modulates Protein Structure and Stability
Navarro, Aaron; Encinar, Jose A.; Lopez-Mendez, Blanca; Aguado-Llera, David; Prieto, Jesus; Gomez, Javier; Alfonso Matinez-Cruz, Luis; Millet, Oscar; Manuel Gonzalez-Ros, Jose; Fernandez-Ballester, ...
BIOCHEMISTRY
2012-04-24
Hydration Dynamics of a Halophilic Protein in Folded and Unfolded States
Qvist, Johan; Ortega, Gabriel; Tadeo, Xavier; Millet, Oscar; Halle, Bertil
JOURNAL OF PHYSICAL CHEMISTRY B
2012-03-15

2017

STRUCTURAL, THERMODYNAMIC, AND MECHANISTICAL STUDIES IN UROPORPHYRINOGEN III SYNTHASE: MOLECULAR BASIS OF CONGENITAL ERYTHROPOIETIC PORPHYRIA
Fortian, Arola; Castano, David; Gonzalez, Esperanza; Lain, Ana; Falcon-Perez, Juan M.; Millet, Oscar
ADVANCES IN PROTEIN CHEMISTRY AND STRUCTURAL BIOLOGY: PROTEIN STRUCTURE AND DISEASES, VOL 83
2017-07-28

2011

N-15 Relaxation NMR Studies of Prolyl Oligopeptidase, an 80 kDa Enzyme, Reveal a Pre-existing Equilibrium between Different Conformational States
Kichik, Nessim; Tarrago, Teresa; Claasen, Birgit; Gairi, Margarida; Millet, Oscar; Giralt, Ernest
CHEMBIOCHEM
2011-12-16
Methionine Adenosyltransferase 1A Gene Deletion Disrupts Hepatic Very Low-Density Lipoprotein Assembly in Mice
Cano, Ainara; Buque, Xabier; Martinez-Una, Maite; Aurrekoetxea, Igor; Menor, Ariane; Garcia-Rodriguez, Juan L.; Lu, Shelly C.; Luz Martinez-Chantar, M.; Mato, Jose M.; Ochoa, Begona; Aspichueta, ...
HEPATOLOGY
2011-12-01
Halophilic enzyme activation induced by salts
Ortega, Gabriel; Lain, Ana; Tadeo, Xavier; Lopez-Mendez, Blanca; Castano, David; Millet, Oscar
SCIENTIFIC REPORTS
2011-06-14
Macromolecular Crowding Fails To Fold a Globular Protein in Cells
Schlesinger, Alexander P.; Wang, Yaqiang; Tadeo, Xavier; Millet, Oscar; Pielak, Gary
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
2011-06-01
Intracellular Rescue of the Uroporphyrinogen III Synthase Activity in Enzymes Carrying the Hotspot Mutation C73R
Fortian, Arola; Gonzalez, Esperanza; Castano, David; Falcon-Perez, Juan M.; Millet, Oscar
JOURNAL OF BIOLOGICAL CHEMISTRY
2011-04-15
Backbone chemical shifts assignments of d-allose binding protein in the free form and in complex with d-allose
Castano, David; Millet, Oscar
BIOMOLECULAR NMR ASSIGNMENTS
2011-04-01

2010

Liquid Chromatography-Mass Spectrometry-Based Parallel Metabolic Profiling of Human and Mouse Model Serum Reveals Putative Biomarkers Associated with the Progression of Nonalcoholic Fatty Liver Disease
Barr, Jonathan; Vazquez-Chantada, Mercedes; Alonso, Cristina; Perez-Cormenzana, Miriam; Mayo, Rebeca; Galan, Asier; Caballeria, Juan; Martin-Duce, Antonio; Tran, Albert; Wagner, Conrad; Luka, ...
JOURNAL OF PROTEOME RESEARCH
2010-09-01
The N-terminal domain of the enzyme I is a monomeric well-folded protein with a low conformational stability and residual structure in the unfolded state
Romero-Beviar, Manuel; Martinez-Rodriguez, Sergio; Prieto, Jesus; Goormaghtigh, Erik; Ariz, Usue; de la Luz Martinez-Chantar, Maria; Gomez, Javier; Neira, Jose L.
PROTEIN ENGINEERING DESIGN & SELECTION
2010-09-01
Fatty Liver and Fibrosis in Glycine N-Methyltransferase Knockout Mice Is Prevented by Nicotinamide
Varela-Rey, Marta; Martinez-Lopez, Nuria; Fernandez-Ramos, David; Embade, Nieves; Calvisi, Diego F.; Woodhoo, Aswhin; Rodriguez, Juan; Fraga, Mario F.; Julve, Josep; Rodriguez-Millan, Elisabeth; ...
HEPATOLOGY
2010-07-01
HuR/Methyl-HuR and AUF1 Regulate the MAT Expressed During Liver Proliferation, Differentiation, and Carcinogenesis
Vazquez-Chantada, Mercedes; Fernandez-Ramos, David; Embade, Nieves; Martinez-Lopez, Nuria; Varela-Rey, Marta; Woodhoo, Ashwin; Luka, Zigmund; Wagner, Conrad; Anglim, Paul P.; Finnell, Richard ...
GASTROENTEROLOGY
2010-05-01
Non-alcoholic fatty liver disease proteomics
Rodriguez-Suarez, Eva; Duce, Antonio M.; Caballeria, Juan; Martinez Arrieta, Felix; Fernandez, Estefania; Gomara, Carolina; Alkorta, Nere; Ariz, Usue; Luz Martinez-Chantar, M.; Lu, Shelly C.; ...
PROTEOMICS CLINICAL APPLICATIONS
2010-04-01
Thermodynamics of protein-cation interaction: Ca+2 and Mg+2 binding to the fifth binding module of the LDL receptor
Arias-Moreno, Xabier; Cuesta-Lopez, Santiago; Millet, Oscar; Sancho, Javier; Velazquez-Campoy, Adrian
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
2010-03-01
Binding of S-Methyl-5 '-Thioadenosine and S-Adenosyl-L-Methionine to Protein MJ0100 Triggers an Open-to-Closed Conformational Change in Its CBS Motif Pair
Lucas, Maria; Antonio Encinar, Jose; Astigarraga Arribas, Egoitz; Oyenarte, Iker; Gomez Garcia, Inmaculada; Kortazar, Danel; Fernandez, Jose A.; Mato, Jose M.; Luz Martinez-Chantar, Maria; Alfonso ...
JOURNAL OF MOLECULAR BIOLOGY
2010-02-26
Diagonal-Free 3D/4D HN,HN-TROSY-NOESY-TROSY
Diercks, Tammo; Truffault, Vincent; Coles, Murray; Millett, Oscar
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
2010-02-24

2009

Structural Basis for the Aminoacid Composition of Proteins from Halophilic Archea
Tadeo, Xavier; Lopez-Mendez, Blanca; Trigueros, Tamara; Lain, Ana; Castano, David; Millet, Oscar
PLOS BIOLOGY
2009-12-01
Protein Stabilization and the Hofmeister Effect: The Role of Hydrophobic Solvation
Tadeo, Xavier; Lopez-Mendez, Blanca; Castano, David; Trigueros, Tamara; Millet, Oscar
BIOPHYSICAL JOURNAL
2009-11-04
Purification, crystallization and preliminary crystallographic analysis of protein MJ1225 from Methanocaldococcus jannaschii, a putative archaeal homologue of gamma-AMPK
Gomez Garcia, Inmaculada; Kortazar, Danel; Oyenarte, Iker; Maria Mato, Jose; Luz Martinez-Chantar, Maria; Alfonso Martinez-Cruz, Luis
ACTA CRYSTALLOGRAPHICA. SECTION F, STRUCTURAL BIOLOGY COMMUNICATIONS
2009-08-01
Impaired Liver Regeneration in Mice Lacking Glycine N-Methyltransferase
Varela-Rey, Marta; Fernandez-Ramos, David; Martinez-Lopez, Nuria; Embade, Nieves; Gomez-Santos, Laura; Beraza, Naiara; Vazquez-Chantada, Mercedes; Rodriguez, Juan; Luka, Zigmund; Wagner, Conrad; ...
HEPATOLOGY
2009-08-01
Phenotypic Differences Between Two Gnmt-/- Mouse Models for Hepatocellular Carcinoma Reply
Luka, Zigmund; Luz Martinez-Chantar, M.; Lu, Shelly C.; Wager, Conrad; Mato, Jose M.
HEPATOLOGY
2009-06-01
The CBS Domain Protein MJ0729 of Methanocaldococcus jannaschii Is a Thermostable Protein with a pH-Dependent Self-Oligomerization
Alfonso Martinez-Cruz, Luis; Encinar, Jose A.; Kortazar, Danel; Prieto, Jesus; Gomez, Javier; Fernandez-Millan, Pablo; Lucas, Maria; Astigarraga Arribas, Egoitz; Andres Fernandez, Jose; Luz ...
BIOCHEMISTRY
2009-03-31
S-Adenosylmethionine Regulates Apurinic/Apyrimidinic Endonuclease 1 Stability: Implication in Hepatocarcinogenesis
Tomasi, Maria Lauda; Iglesias-Ara, Ainhoa; Yang, Heping; Ramani, Komal; Feo, Francesco; Pascale, Maria Rosa; Luz Martinez-Chantar, M.; Mato, Jose M.; Lu, Shelly C.
GASTROENTEROLOGY
2009-03-01
Evidence for LKB1/AMP-Activated Protein Kinase/Endothelial Nitric Oxide Synthase Cascade Regulated by Hepatocyte Growth Factor, S-Adenosylmethionine, and Nitric Oxide in Hepatocyte Proliferation
Vazquez-Chantada, Mercedes; Ariz, Usue; Varela-Rey, Marta; Embade, Nieves; Martinez-Lopez, Nuria; Fernandez-Ramos, David; Gomez-Santos, Laura; Lamas, Santiago; Lu, Shelly C.; Luz Martinez-Chantar, ...
HEPATOLOGY
2009-02-01
Uroporphyrinogen III Synthase Mutations Related to Congenital Erythropoietic Porphyria Identify a Key Helix for Protein Stability
Fortian, Arola; Castano, David; Ortega, Gabriel; Lain, Ana; Pons, Miquel; Millet, Oscar
BIOCHEMISTRY
2009-01-20

2007

Anion modulation of the H-1/H-2 exchange rates in backbone amide protons monitored by NMR spectroscopy
Tadeo, Xavier; Castano, David; Millet, Oscar
PROTEIN SCIENCE
2007-12-01
Influence of the hofmeister anions on protein stability as studied by thermal denaturation and chemical shift perturbation
Tadeo, Xavier; Pons, Miquel; Millet, Oscar
BIOCHEMISTRY
2007-01-23

Protein stability (thermodynamic and kinetic) drives the biophysical properties of the polypeptide chain (protein folding) and the protein's concentration in the cellular environment (protein homeostasis). It is the result of a delicate balance between inter- and intramolecular interactions, which can be easily altered by mutations and/or upon changes in the composition of the surrounding media. In this context, NMR spectroscopy offers a plethora of suitable experiments to investigate protein stability. In our laboratory we are currently interested in the following topics:

  • Pharmacological chaperones. Rare diseases (~7000 identified to date) are an area of significant medical need affecting an estimated 350 million people worldwide, with ~95% having no currently approved drug treatment. They are often produced by inherited mutations affecting the activity of a protein and It is becoming increasily clear that, most frequently, a mutation destabilizes the protein/enzyme, ultimately affecting its intracellular homeostasis. In this context, pharmacological chaperones (small molecules which bind to the protein, restoring stability and activity without affecting its function) can be applied to many diseases. In our laboratory we are investigating new methods (NMR, biophysical and biochemical) for the discovery and characterization of pharmacological chaperones against a set of diseases: congenital eryhtropoietic porphyria, tyrosianemia.
     
  • Environmental modulation of enzyme stability. The high catalytic efficiency and the exquisite enantioselectivity of an enzyme has been employed in some industrial processes to upgrade their properties in order to make them more environmentally-friendly. However, large scale industrial implementation of biotechnological reactions is often limited by the marginal stability of the enzyme in the reactor conditions. In our laboratory we employ NMR and circular dichroism to investigate the effect of external crowding agents to improve the activity and stability of several enzymes. Specifically, we are investigating the mechanism for protein haloadaptation by a combined use of site directed mutagenesis and high-resolution NMR spectroscopy.
Latest Publications

Targeting Hepatic Glutaminase 1 Ameliorates Non-alcoholic Steatohepatitis by Restoring Very-Low-Density Lipoprotein Triglyceride Assembly

Simon, J;Nunez-Garcia, M;Fernandez-Tussy, P;Barbier-Torres, L;Fernandez-Ramos, D;Gomez-Santos, B;Buque, X;Lopitz-Otsoa, F;Goikoetxea-Usandizaga, N;Serrano-Macia, M;Rodriguez-Agudo, R;Bizkarguenaga, ...

CELL METABOLISM

2020-03-03

Methionine Adenosyltransferase alpha 1 Is Targeted to the Mitochondrial Matrix and Interacts with Cytochrome P450 2E1 to Lower Its Expression

Murray, B;Peng, H;Barbier-Torres, L;Robinson, AE;Li, TWH;Fan, W;Tomasi, ML;Gottlieb, RA;Van Eyk, J;Lu, ZM;Mart?nez-Chantar, ML;Liangpunsakul, S;Skill, NJ;Mato, JM;Lu, SC

Hepatology (Baltimore, Md.)

2019-12-01

miR-873-5p targets mitochondrial GNMT-Complex II interface contributing to non-alcoholic fatty liver disease

Fernandez-Tussy, P;Fernandez-Ramos, D;Lopitz-Otsoa, F;Simon, J;Barbier-Torres, L;Gomez-Santos, B;Nunez-Garcia, M;Azkargorta, M;Gutierrez-de Juan, V;Serrano-Macia, M;Rodriguez-Agudo, R;Iruzubieta, ...

MOLECULAR METABOLISM

2019-11-01

Methionine adenosyltransferases in liver cancer

Murray, B;Barbier-Torres, L;Fan, W;Mato, JM;Lu, SC

WORLD JOURNAL OF GASTROENTEROLOGY

2019-08-21

Biomarkers and subtypes of deranged lipid metabolism in non-alcoholic fatty liver disease

Mato, JM;Alonso, C;Noureddin, M;Lu, SC

WORLD JOURNAL OF GASTROENTEROLOGY

2019-06-28

Control and regulation of S-Adenosylmethionine biosynthesis by the regulatory beta subunit and quinolone-based compounds

Panmanee, J;Bradley-Clarke, J;Mato, JM;O'Neill, PM;Antonyuk, SV;Hasnain, SS

FEBS JOURNAL

2019-06-01

Metabolomics discloses potential biomarkers to predict the acute HVPG response to propranolol in patients with cirrhosis

Reverter, E;Lozano, JJ;Alonso, C;Berzigotti, A;Seijo, S;Turon, F;Baiges, A;Martinez-Chantar, ML;Mato, JM;Martinez-Arranz, I;La Mura, V;Hernandez-Gea, V;Bosch, J;Garcia-Pagan, JC

LIVER INTERNATIONAL

2019-04-01

NEDDylation inhibition as a new potential therapy of nonalcoholic fatty liver disease

Serrano-Macia, M;Azkargorta, M;Simon, J;Otsoa, FL;Delgado, TC;Ramos, DF;de Juan, VG;Varela-Rey, M;Goikoetxea, N;Tussy, PF;Apichueta, P;de Urturi, DS;Buque, X;Iruzubieta, P;Crespo, J;Lu, SC;Mato, ...

JOURNAL OF HEPATOLOGY

2019-04-01

Mitochondrial GNMT-complex II is recovered by miR-873-5p targeting in NAFLD

Ramos, DF;Tussy, PF;Otsoa, FL;Simon, J;Torres, LB;Santos, BG;Nunez, M;Azkargorta, M;de Juan, VG;Serrano-Macia, M;Agudo, RR;Champagne, DG;Iruzubieta, P;Anguita, J;Rincon, M;Elortza, F;Anita, ...

JOURNAL OF HEPATOLOGY

2019-04-01

Aramchol, SCD1 inhibitor, improves liver glucose homeostasis in NASH

Mato, JM;delaCruz-Villar, L;Fernandez-Ramos, D;Lopitz, F;Azkargorta, M;Elortza, F;Iruarrizaga-Lejarreta, M;Alonso, C;Barbier-Torres, L;Lu, SC;Hayardeny, L;Gorfine, T;Sanyal, AJ

JOURNAL OF HEPATOLOGY

2019-04-01