Research Support Facilities

Monoclonal Antibody Facility

Welcome

The Monoclonal Antibody Service provides consultation and the services to generate monoclonal antibodies against a diverse array of antigens (proteins, peptides, glycoproteins, etc.) and their characterization by ELISA, Western blotting or immunohistochemistry. Our antibody generation facility is a member of EuroMAbNet. Check out the many resources available : www.euromabnet.com

Instrumentation

  • Immunization: Two mice are immunized following standard procedures unless there is a specific need. The mice are immunized using Complete Freund's adjuvant, followed by two boosts in Incomplete Freund's adjuvant. Before the second boost, the sera are tested for reactivity to the antigen.
  • Fusion: Spleen cells are fused with the 0-Ag14 cell line and selected for hybridomas using HAT selective medium. The hybridomas are then cloned.
  • Characterization: Individual clone supernatants are then tested for antibodies against the antigen of interest by ELISA. Positive clones are further tested by Western blotting and immunohistochemistry against a panel of murine or human tissues, when appropriate.

Service catalogue

We are highly specialised in the production of mouse monoclonal antibodies. It is also offered mAb characterisation and validation and mediumscale mAb production.

Complete catalogue of available antibodies will be available soon.

For more information follow this link

Service Request

For information about experimental design and quote contact directly Juan Anguita through our dedicated tool. link