IMI2 Programme
0000-0003-1264-3153
0000-0001-8748-4105José M Mato is founder and General Director of the Centers bioGUNE (Bilbao) and biomaGUNE (San Sebastián), and Research Professor of the Spanish National Research Council (CSIC), Spain. A graduate in Biochemistry at the Complutense University of Madrid, Professor Mato received a PhD degree from Leiden University and was awarded the CJ Kok prize for his thesis. He was a postdoctoral fellow at the Biozentrum of the University of Basel and the National Institutes of Health, and a faculty member at the Jiménez Díaz Foundation in Madrid before been named Research Professor at the CSIC. He has been Professor of the Faculty of Medicine of the University of Navarra and Visiting Professor at the University of Pennsylvania and Thomas Jeff erson University. From 1992 to 1996 Professor Mato was President of the CSIC and in 2004 was awarded the Spanish National Research Prize in Medicine.
Latest Publications
2023
2022
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Natxo Reguera Pascual
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Begoña Bareño
Members
TECHNICIANS / DOCTORAL CANDIDATESProtein stability (thermodynamic and kinetic) drives the biophysical properties of the polypeptide chain (protein folding) and the protein's concentration in the cellular environment (protein homeostasis). It is the result of a delicate balance between inter- and intramolecular interactions, which can be easily altered by mutations and/or upon changes in the composition of the surrounding media. In this context, NMR spectroscopy offers a plethora of suitable experiments to investigate protein stability. In our laboratory we are currently interested in the following topics:
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Pharmacological chaperones. Rare diseases (~7000 identified to date) are an area of significant medical need affecting an estimated 350 million people worldwide, with ~95% having no currently approved drug treatment. They are often produced by inherited mutations affecting the activity of a protein and It is becoming increasily clear that, most frequently, a mutation destabilizes the protein/enzyme, ultimately affecting its intracellular homeostasis. In this context, pharmacological chaperones (small molecules which bind to the protein, restoring stability and activity without affecting its function) can be applied to many diseases. In our laboratory we are investigating new methods (NMR, biophysical and biochemical) for the discovery and characterization of pharmacological chaperones against a set of diseases: congenital eryhtropoietic porphyria, tyrosianemia.
- Environmental modulation of enzyme stability. The high catalytic efficiency and the exquisite enantioselectivity of an enzyme has been employed in some industrial processes to upgrade their properties in order to make them more environmentally-friendly. However, large scale industrial implementation of biotechnological reactions is often limited by the marginal stability of the enzyme in the reactor conditions. In our laboratory we employ NMR and circular dichroism to investigate the effect of external crowding agents to improve the activity and stability of several enzymes. Specifically, we are investigating the mechanism for protein haloadaptation by a combined use of site directed mutagenesis and high-resolution NMR spectroscopy.
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Group Leader
Óscar Millet
Emerging Scientist -
Gabriel Ortega Quintanilla
Ikerbasque Research Fellow - Ramón y Cajal (RyC) Programme RESEARCH ASSISTANT -
Nieves Embade
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Fernando Lopitz Otsoa
SPECIALIST -
Rubén Gil Redondo
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Beatriz González Valle
POSTDOCTORAL RESEARCHER -
Ganeko Bernardo
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Alain Ibañez de Opakua Lopez de Abetxuko
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Andreas Schedlbauer
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David Fernández Ramos
TECHNICIANS / DOCTORAL CANDIDATES -
Maider Bizkarguenaga Uribiarte
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Virginia Gutiérrez de Juan
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Uxue Hermoso Martínez
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Riccardo Scarin
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Sara Martín Ramos
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Alba Pejenaute Pejenaute
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Laura Gálvez Larrosa
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Karen Salua Alarcón Morales
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Maria de los Reyes Luque Urbano
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Cristina Garrido
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Ángela de Diego Rodríguez
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Pablo Herrero Alfonso
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Margarita Gómez Galán
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Ricardo Diogo Alves Conde
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Tania Pereira Ortuzar
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Jon Gil Martinez
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Ana Laín
Members
Latest Publications
Stratification of Sepsis Patients on Admission into the Intensive Care Unit According to Differential Plasma Metabolic Phenotypes
Lodge, S; Litton, E; Gray, N; Ryan, M; Millet, O; Fear, M; Raby, E; Currie, A; Wood, F; Holmes, E; Wist, J; Nicholson, JK;
JOURNAL OF PROTEOME RESEARCH
2024-03-21
Cross-Validation of Metabolic Phenotypes in SARS-CoV-2 Infected Subpopulations Using Targeted Liquid Chromatography-Mass Spectrometry (LC-MS)
Whiley, L; Lawler, NG; Zeng, AX; Lee, A; Chin, ST; Bizkarguenaga, M; Bruzzone, C; Embade, N; Wist, J; Holmes, E; Millet, O; Nicholson, JK; Gray, N;
JOURNAL OF PROTEOME RESEARCH
2024-03-14
Characterization of preovulatory follicular fluid secretome and its effects on equine oocytes during in vitro maturation.
Luis-Calero, Marcos; Marinaro, Federica; Fernandez-Hernandez, Pablo; Ortiz-Rodriguez, Jose M; G Casado, Javier; Pericuesta, Eva; Gutierrez-Adan, Alfonso; Gonzalez, Esperanza; Azkargorta, Mikel; ...
Research in veterinary science
2024-03-11
Quantitative Analysis of the Human Semen Phosphorometabolome by 31P-NMR
Serrano, R; Martin-Hidalgo, D; Bilbao, J; Bernardo-Seisdedos, G; Millet, O; Garcia-Marin, LJ; Bragado, MJ;
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
2024-02-01
Effect of age and dietary habits on Red Blood Cell membrane fatty acids in a Southern Europe population (Basque Country)
Marrugat, G; Cano, A; Amézaga, J; Arranz, S; Embade, N; Millet, O; Ferreri, C; Tueros, I;
PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS
2023-12-25
Hepatocyte-specific O-GlcNAc transferase downregulation ameliorates nonalcoholic steatohepatitis by improving mitochondrial function
Gonzalez-Rellan, MJ; Parracho, T; Heras, V; Rodriguez, A; Fondevila, MF; Novoa, E; Lima, N; Varela-Rey, M; Senra, A; Chantada-Vazquez, MDP; Ameneiro, C; Bernardo, G; Fernandez-Ramos, D; Lopitz-Otsoa, ...
MOLECULAR METABOLISM
2023-09-01
Hepatic levels of S-adenosylmethionine regulate the adaptive response to fasting
Capelo-Diz, A; Lachiondo-Ortega, S; Fernández-Ramos, D; Cañas-Martín, J; Goikoetxea-Usandizaga, N; Serrano-Maciá, M; González-Rellan, MJ; Mosca, L; Blazquez-Vicens, J; Tinahones-Ruano, A; Fondevila, ...
CELL METABOLISM
2023-08-08
Serum and Urine Metabolomic Profiling of Newly Diagnosed Treatment-Naive Inflammatory Bowel Disease Patients
Aldars-García, L; Gil-Redondo, R; Embade, N; Riestra, S; Rivero, M; Gutiérrez, A; Rodríguez-Lago, I; Fernández-Salazar, L; Ceballos, D; Benitez, JM; Aguas, M; Baston-Rey, I; Bermejo, F; Casanova, ...
INFLAMMATORY BOWEL DISEASES
2023-08-03
Performance of non-invasive tests and histology for the prediction of clinical outcomes in patients with non-alcoholic fatty liver disease: an individual participant data meta-analysis
Mózes, FE; Lee, JA; Vali, Y; Alzoubi, O; Staufer, K; Trauner, M; Paternostro, R; Stauber, RE; Holleboom, AG; van Dijk, AM; Mak, AL; Boursier, J; Loup, MD; Shima, T; Bugianesi, E; Gaia, S; Armandi, ...
Lancet Gastroenterology & Hepatology
2023-08-01
Integrative Plasma Metabolic and Lipidomic Modelling of SARS-CoV-2 Infection in Relation to Clinical Severity and Early Mortality Prediction
Lodge, S; Lawler, NG; Gray, N; Masuda, R; Nitschke, P; Whiley, L; Bong, SH; Yeap, BB; Dwivedi, G; Spraul, M; Schaefer, H; Gil-Redondo, R; Embade, N; Millet, O; Holmes, E; Wist, J; Nicholson, ...
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
2023-07-01
Urinary Metabolomic Profile of Youth at Risk of Chronic Kidney Disease in Nicaragua
Hall, SM; Raines, NH; Ramirez-Rubio, O; Amador, JJ; López-Pilarte, D; OCallaghan-Gordo, C; Gil-Redondo, R; Embade, N; Millet, O; Peng, XJ; Vences, S; Keogh, SA; Delgado, IS; Friedman, DJ; Brooks, ...
KIDNEY360
2023-07-01
Metabolic Features of Increased Gut Permeability, Inflammation, and Altered Energy Metabolism Distinguish Agricultural Workers at Risk for Mesoamerican Nephropathy
Raines, NH; Leone, DA; OCallaghan-Gordo, C; Ramirez-Rubio, O; Amador, JJ; Pilarte, DL; Delgado, IS; Leibler, JH; Embade, N; Gil-Redondo, R; Bruzzone, C; Bizkarguenaga, M; Scammell, MK; Parikh, ...
METABOLITES
2023-03-01
Redox regulation of KV7 channels through EF3 hand of calmodulin
Nunez, E; Jones, F; Muguruza-Montero, A; Urrutia, J; Aguado, A; Malo, C; Bernardo-Seisdedos, G; Domene, C; Millet, O; Gamper, N; Villarroel, A; Colecraft, HM;
ELIFE
2023-02-20
Hyperphosphorylation of hepatic proteome characterizes nonalcoholic fatty liver disease in S-adenosylmethionine deficiency
Robinson, AE; Binek, A; Ramani, K; Sundararaman, N; Barbier-Torres, L; Murray, B; Venkatraman, V; Kreimer, S; Mc Ardle, A; Noureddin, M; Fernández-Ramos, D; Lopitz-Otsoa, F; de Juan, VG; Millet, ...
ISCIENCE
2023-02-17
Metabolomics as a powerful tool for diagnostic, pronostic and drug intervention analysis in COVID-19
Bruzzone, C; Conde, R; Embade, N; Mato, JM; Millet, O;
FRONTIERS IN MOLECULAR BIOSCIENCES
2023-02-15
Protoporphyrin IX Binds to Iron(II)-Loaded and to Zinc-Loaded Human Frataxin
Bernardo-Seisdedos, G; Schedlbauer, A; Pereira-Ortuzar, T; Mato, JM; Millet, O;
LIFE-BASEL
2023-01-01
NMR Investigation of Protein-Carbohydrate Interactions: The Recognition of Glycans by Galectins Engineered with Fluorotryptophan Residues
Lete, MG; Franconetti, A; Bertuzzi, S; Delgado, S; Azkargorta, M; Elortza, F; Millet, O; Jiménez-Osés, G; Arda, A; Jiménez-Barbero, J;
CHEMISTRY-A EUROPEAN JOURNAL
2022-12-12
An NMR-Based Model to Investigate the Metabolic Phenoreversion of COVID-19 Patients throughout a Longitudinal Study
Gil-Redondo, R; Conde, R; Bizkarguenaga, M; Bruzzone, C; Laín, A; González-Valle, B; Iriberri, M; Ramos-Acosta, C; Anguita, E; Lariz, JIA; Yandiola, PPE; Moran, MA; Jiménez-Mercado, ME; Egia-Mendikute, ...
METABOLITES
2022-12-01
A flow cytometry-based neutralization assay for simultaneous evaluation of blocking antibodies against SARS-CoV-2 variants
Egia-Mendikute, L; Bosch, A; Prieto-Fernández, E; Vila-Vecilla, L; Zanetti, SR; Lee, SY; Jiménez-Lasheras, B; del Río, AG; Antoñana-Vildosola, A; de Blas, A; Velasco-Beltrán, P; Serrano-Maciá, ...
FRONTIERS IN IMMUNOLOGY
2022-11-24
A glutamine-based single α-helix scaffold to target globular proteins
Escobedo, A; Piccirillo, J; Aranda, J; Diercks, T; Mateos, B; Garcia-Cabau, C; Sánchez-Navarro, M; Topal, B; Biesaga, M; Staby, L; Kragelund, BB; García, J; Millet, O; Orozco, M; Coles, M; Crehuet, ...
NATURE COMMUNICATIONS
2022-11-18