Ganeko Bernardo
gbernardo
Ganeko Bernardo
POST DOCTORAL RESEARCHER
Precision Medicine and Metabolism Lab
Email: gbernardo.atlas@cicbiogune.es
Phone: 4452
Address: Bizkaia Science and Technology Park, building 800, Derio (Bizkaia)
Latest Publications
Metabolic Landscape of the Mouse Liver by Quantitative P-31 Nuclear Magnetic Resonance Analysis of the Phosphorome
Bernardo-Seisdedos, G;Bilbao, J;Fernandez-Ramos, D;Lopitz-Otsoa, F;de Juan, VG;Bizkarguenaga, M;Mateos, B;Fondevila, MF;Abril-Fornaguera, J;Diercks, T;Lu, SC;Nogueiras, R;Mato, JM;Millet, O
HEPATOLOGY
2021-06-15
Identification of novel UROS mutations in a patient with congenital erythropoietic porphyria and efficient treatment by phlebotomy
Blouin, JM;Ged, C;Bernardo-Seisdedos, G;Cabantous, T;Pinson, B;Poli, A;Puy, H;Millet, O;Gouya, L;Morice-Picard, F;Richard, E
MOLECULAR GENETICS AND METABOLISM REPORTS
2021-06-01
Improving the Pharmacological Properties of Ciclopirox for Its Use in Congenital Erythropoietic Porphyria
Bernardo-Seisdedos, G;Charco, JM;SanJuan, I;Garcia-Martinez, S;Urquiza, P;Erana, H;Castilla, J;Millet, O
JOURNAL OF PERSONALIZED MEDICINE
2021-06-01
Cosolute modulation of protein oligomerization reactions in the homeostatic timescale
Mateos, B;Bernardo-Seisdedos, G;Dietrich, V;Zalba, N;Ortega, G;Peccati, F;Jimenez-Oses, G;Konrat, R;Tollinger, M;Millet, O
BIOPHYSICAL JOURNAL
2021-05-18
Therapeutic Targeting of Fumaryl Acetoacetate Hydrolase in Hereditary Tyrosinemia Type I
Gil-Martinez, J;Macias, I;Unione, L;Bernardo-Seisdedos, G;Lopitz-Otsoa, F;Fernandez-Ramos, D;Lain, A;Sanz-Parra, A;Mato, JM;Millet, O
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
2021-02-01

2020

Unravelling the Time Scale of Conformational Plasticity and Allostery in Glycan Recognition by Human Galectin-1
Bertuzzi, S;Gimeno, A;Nunez-Franco, R;Bernardo-Seisdedos, G;Delgado, S;Jimenez-Oses, G;Millet, O;Jimenez-Barbero, J;Arda, A
CHEMISTRY-A EUROPEAN JOURNAL
2020-10-29
SARS-CoV-2 Infection Dysregulates the Metabolomic and Lipidomic Profiles of Serum
Bruzzone, C;Bizkarguenaga, M;Gil-Redondo, R;Diercks, T;Arana, E;de Vicuna, AG;Seco, M;Bosch, A;Palazon, A;San Juan, I;Lain, A;Gil-Martinez, J;Bernardo-Seisdedos, G;Fernandez-Ramos, D;Lopitz-Otsoa, ...
ISCIENCE
2020-10-23
Abnormal concentration of porphyrins in serum from COVID-19 patients
San Juan, I;Bruzzone, C;Bizkarguenaga, M;Bernardo-Seisdedos, G;Lain, A;Gil-Redondo, R;Diercks, T;Gil-Martinez, J;Urquiza, P;Arana, E;Seco, M;de Vicuna, AG;Embade, N;Mato, JM;Millet, O
BRITISH JOURNAL OF HAEMATOLOGY
2020-08-29
An Epoxide Intermediate in Glycosidase Catalysis
Sobala, LF;Speciale, G;Zhu, S;Raich, L;Sannikova, N;Thompson, AJ;Hakki, Z;Lu, D;Abadi, SSK;Lewis, AR;Rojas-Cervellera, V;Bernardo-Seisdedos, G;Zhang, YM;Millet, O;Jimenez-Barbero, J;Bennet, ...
ACS CENTRAL SCIENCE
2020-05-27

2019

Hereditary tyrosinemia type I-associated mutations in fumarylacetoacetate hydrolase reduce the enzyme stability and increase its aggregation rate
Macias, I;Lain, A;Bernardo-Seisdedos, G;Gil, D;Gonzalez, E;Falcon-Perez, JM;Millet, O
JOURNAL OF BIOLOGICAL CHEMISTRY
2019-08-30
Side chain to main chain hydrogen bonds stabilize a polyglutamine helix in a transcription factor
Escobedo, A;Topal, B;Kunze, MBA;Aranda, J;Chiesa, G;Mungianu, D;Bernardo-Seisdedos, G;Eftekharzadeh, B;Gairi, M;Pierattelli, R;Felli, IC;Diercks, T;Millet, O;Garcia, J;Orozco, M;Crehuet, R;Lindorff-Larsen, ...
NATURE COMMUNICATIONS
2019-05-02

2018

From 1,4-Disaccharide to 1,3-Glycosyl Carbasugar: Synthesis of a Bespoke Inhibitor of Family GH99 Endo-alpha-mannosidase
Lu, D;Zhu, S;Sobala, LF;Bernardo-Seisdedos, G;Milet, O;Zhang, YM;Jimenez-Barbero, J;Davies, GJ;Sollogoub, M
ORGANIC LETTERS
2018-12-07
Repurposing ciclopirox as a pharmacological chaperone in a model of congenital erythropoietic porphyria
Urquiza, P;Lain, A;Sanz-Parra, A;Moreno, J;Bernardo-Seisdedos, G;Dubus, P;Gonzalez, E;Gutierrez-de-Juan, V;Garcia, S;Erana, H;Juan, IS;Macias, I;Ben Bdira, F;Pluta, P;Ortega, G;Oyarzabal, J;Gonzalez-Muniz, ...
SCIENCE TRANSLATIONAL MEDICINE
2018-09-19
Structural basis of pyrrole polymerization in human porphobilinogen deaminase
Pluta, P;Roversi, P;Bernardo-Seisdedos, G;Rojas, AL;Cooper, JB;Gu, S;Pickersgill, RW;Millet, O
BIOCHIMICA ET BIOPHYSICA ACTA
2018-09-01
Structural basis and energy landscape for the Ca2+ gating and calmodulation of the Kv7.2 K+ channel
Bernardo-Seisdedos, G;Nunez, E;Gomis, C;Malo, C;Villarroel, A;Millet, O
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
2018-03-06

2017

Disulfide driven folding for a conditionally disordered protein
Fraga, H;Pujols, J;Gil-Garcia, M;Roque, A;Bernardo-Seisdedos, G;Santambrogio, C;Bech-Serra, JJ;Canals, F;Bernado, P;Grandori, R;Millet, O;Ventura, S
SCIENTIFIC REPORTS
2017-12-05
Missense UROS mutations causing congenital erythropoietic porphyria reduce UROS homeostasis that can be rescued by proteasome inhibition
Blouin, JM;Bernardo-Seisdedos, G;Sasso, E;Esteve, J;Ged, C;Lalanne, M;Sanz-Parra, A;Urquiza, P;de Verneuil, H;Millet, O;Richard, E
HUMAN MOLECULAR GENETICS
2017-04-15
Contribution of Shape and Charge to the Inhibition of a Family GH99 endo-alpha-1,2-Mannanase
Petricevic, M;Sobala, LF;Fernandes, PZ;Raich, L;Thompson, AJ;Bernardo-Seisdedos, G;Millet, O;Zhu, S;Sollogoub, M;Jimenez-Barbero, J;Rovira, C;Davies, GJ;Williams, SJ
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
2017-01-25

2014

Pivoting between Calmodulin Lobes Triggered by Calcium in the Kv7.2/Calmodulin Complex
Alaimo, A;Alberdi, A;Gomis-Perez, C;Fernandez-Orth, J;Bernardo-Seisdedos, G;Malo, C;Millet, O;Areso, P;Villarroel, A
PLOS ONE
2014-01-28

Protein stability (thermodynamic and kinetic) drives the biophysical properties of the polypeptide chain (protein folding) and the protein's concentration in the cellular environment (protein homeostasis). It is the result of a delicate balance between inter- and intramolecular interactions, which can be easily altered by mutations and/or upon changes in the composition of the surrounding media. In this context, NMR spectroscopy offers a plethora of suitable experiments to investigate protein stability. In our laboratory we are currently interested in the following topics:

  • Pharmacological chaperones. Rare diseases (~7000 identified to date) are an area of significant medical need affecting an estimated 350 million people worldwide, with ~95% having no currently approved drug treatment. They are often produced by inherited mutations affecting the activity of a protein and It is becoming increasily clear that, most frequently, a mutation destabilizes the protein/enzyme, ultimately affecting its intracellular homeostasis. In this context, pharmacological chaperones (small molecules which bind to the protein, restoring stability and activity without affecting its function) can be applied to many diseases. In our laboratory we are investigating new methods (NMR, biophysical and biochemical) for the discovery and characterization of pharmacological chaperones against a set of diseases: congenital eryhtropoietic porphyria, tyrosianemia.
     
  • Environmental modulation of enzyme stability. The high catalytic efficiency and the exquisite enantioselectivity of an enzyme has been employed in some industrial processes to upgrade their properties in order to make them more environmentally-friendly. However, large scale industrial implementation of biotechnological reactions is often limited by the marginal stability of the enzyme in the reactor conditions. In our laboratory we employ NMR and circular dichroism to investigate the effect of external crowding agents to improve the activity and stability of several enzymes. Specifically, we are investigating the mechanism for protein haloadaptation by a combined use of site directed mutagenesis and high-resolution NMR spectroscopy.

Protocolos de Histología

Latest Publications

Uneven metabolic and lipidomic profiles in recovered COVID-19 patients as investigated by plasma NMR metabolomics

Bizkarguenaga, M;Bruzzone, C;Gil-Redondo, R;SanJuan, I;Martin-Ruiz, I;Barriales, D;Palacios, A;Pasco, ST;Gonzalez-Valle, B;Lain, A;Herrera, L;Azkarate, A;Vesga, MA;Eguizabal, C;Anguita, J;Embade, ...

NMR IN BIOMEDICINE

2021-10-27

Metabolomics and lipidomics in NAFLD: biomarkers and non-invasive diagnostic tests

Masoodi, M;Gastaldelli, A;Hyotylainen, T;Arretxe, E;Alonso, C;Gaggini, M;Brosnan, J;Anstee, QM;Millet, O;Ortiz, P;Mato, JM;Dufour, JF;Oresic, M

NATURE REVIEWS GASTROENTEROLOGY & HEPATOLOGY

2021-09-10

O-GlcNAcylated p53 in the liver modulates hepatic glucose production

Gonzalez-Rellan, MJ;Fondevila, MF;Fernandez, U;Rodriguez, A;Varela-Rey, M;Veyrat-Durebex, C;Seoane, S;Bernardo, G;Lopitz-Otsoa, F;Fernandez-Ramos, D;Bilbao, J;Iglesias, C;Novoa, E;Ameneiro, ...

NATURE COMMUNICATIONS

2021-08-20

Integrative Modeling of Plasma Metabolic and Lipoprotein Biomarkers of SARS-CoV-2 Infection in Spanish and Australian COVID-19 Patient Cohorts

Masuda, R;Lodge, S;Nitschke, P;Spraul, M;Schaefer, H;Bong, SH;Kimhofer, T;Hall, D;Loo, RL;Bizkarguenaga, M;Bruzzone, C;Gil-Redondo, R;Embade, N;Mato, JM;Holmes, E;Wist, J;Millet, O;Nicholson, ...

JOURNAL OF PROTEOME RESEARCH

2021-08-06

Deregulated 14-3-3 zeta and methionine adenosyltransferase alpha 1 interplay promotes liver cancer tumorigenesis in mice and humans

Lu, LQ;Zhang, J;Fan, W;Li, Y;Wang, JH;Li, TWH;Torres, LB;Mato, JM;Liu, T;Seki, E;Matsuda, M;Tomasi, ML;Bhowmick, NA;Yang, HP;Lu, SC

ONCOGENE

2021-08-04

A molecular signature for the metabolic syndrome by urine metabolomics

Bruzzone, C;Gil-Redondo, R;Seco, M;Barragan, R;de la Cruz, L;Cannet, C;Schafer, H;Fang, F;Diercks, T;Bizkarguenaga, M;Gonzalez-Valle, B;Lain, A;Sanz-Parra, A;Coltell, O;de Letona, AL;Spraul, ...

CARDIOVASCULAR DIABETOLOGY

2021-07-28

Metabolic Landscape of the Mouse Liver by Quantitative P-31 Nuclear Magnetic Resonance Analysis of the Phosphorome

Bernardo-Seisdedos, G;Bilbao, J;Fernandez-Ramos, D;Lopitz-Otsoa, F;de Juan, VG;Bizkarguenaga, M;Mateos, B;Fondevila, MF;Abril-Fornaguera, J;Diercks, T;Lu, SC;Nogueiras, R;Mato, JM;Millet, O

HEPATOLOGY

2021-06-15

Aramchol downregulates stearoyl CoA-desaturase 1 in hepatic stellate cells to attenuate cellular fibrogenesis

Bhattacharya, D;Basta, B;Mato, JM;Craig, A;Fernandez-Ramos, D;Lopitz-Otsoa, F;Tsvirkun, D;Hayardeny, L;Chandar, V;Schwartz, RE;Villanueva, A;Friedman, SL

JHEP REPORTS

2021-06-01

Anti-miR-518d-5p overcomes liver tumor cell death resistance through mitochondrial activity

Fernandez-Tussy, P;Rodriguez-Agudo, R;Fernandez-Ramos, D;Barbier-Torres, L;Zubiete-Franco, I;de Davalillo, SL;Herraez, E;Goikoetxea-Usandizaga, N;Lachiondo-Ortega, S;Simon, J;Lopitz-Otsoa, F;Gutierrez-de ...

CELL DEATH & DISEASE

2021-05-28

Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals

Egia-Mendikute, L;Bosch, A;Prieto-Fernandez, E;Lee, SY;Jimenez-Lasheras, B;del Rio, AG;Antonana-Vildosola, A;Bruzzone, C;Bizkarguenaga, M;Embade, N;Gil-Redondo, R;Martinez-Chantar, ML;Lopez-Hoyos, ...

COMMUNICATIONS BIOLOGY

2021-04-20