Ganeko Bernardo
gbernardo
Ganeko Bernardo
POST DOCTORAL RESEARCHER
Precision Medicine and Metabolism Lab
Email: gbernardo.atlas@cicbiogune.es
Phone: 4332
Address: Bizkaia Science and Technology Park, building 800, Derio (Bizkaia)
Latest Publications
Unravelling the Time Scale of Conformational Plasticity and Allostery in Glycan Recognition by Human Galectin-1
Bertuzzi, S;Gimeno, A;Nunez-Franco, R;Bernardo-Seisdedos, G;Delgado, S;Jimenez-Oses, G;Millet, O;Jimenez-Barbero, J;Arda, A
CHEMISTRY-A EUROPEAN JOURNAL
2020-10-29
SARS-CoV-2 Infection Dysregulates the Metabolomic and Lipidomic Profiles of Serum
Bruzzone, C;Bizkarguenaga, M;Gil-Redondo, R;Diercks, T;Arana, E;de Vicuna, AG;Seco, M;Bosch, A;Palazon, A;San Juan, I;Lain, A;Gil-Martinez, J;Bernardo-Seisdedos, G;Fernandez-Ramos, D;Lopitz-Otsoa, ...
ISCIENCE
2020-10-23
Abnormal concentration of porphyrins in serum from COVID-19 patients
San Juan, I;Bruzzone, C;Bizkarguenaga, M;Bernardo-Seisdedos, G;Lain, A;Gil-Redondo, R;Diercks, T;Gil-Martinez, J;Urquiza, P;Arana, E;Seco, M;de Vicuna, AG;Embade, N;Mato, JM;Millet, O
BRITISH JOURNAL OF HAEMATOLOGY
2020-08-29
An Epoxide Intermediate in Glycosidase Catalysis
Sobala, LF;Speciale, G;Zhu, S;Raich, L;Sannikova, N;Thompson, AJ;Hakki, Z;Lu, D;Abadi, SSK;Lewis, AR;Rojas-Cervellera, V;Bernardo-Seisdedos, G;Zhang, YM;Millet, O;Jimenez-Barbero, J;Bennet, ...
ACS CENTRAL SCIENCE
2020-05-27

2019

Hereditary tyrosinemia type I-associated mutations in fumarylacetoacetate hydrolase reduce the enzyme stability and increase its aggregation rate
Macias, I;Lain, A;Bernardo-Seisdedos, G;Gil, D;Gonzalez, E;Falcon-Perez, JM;Millet, O
JOURNAL OF BIOLOGICAL CHEMISTRY
2019-08-30
Side chain to main chain hydrogen bonds stabilize a polyglutamine helix in a transcription factor
Escobedo, A;Topal, B;Kunze, MBA;Aranda, J;Chiesa, G;Mungianu, D;Bernardo-Seisdedos, G;Eftekharzadeh, B;Gairi, M;Pierattelli, R;Felli, IC;Diercks, T;Millet, O;Garcia, J;Orozco, M;Crehuet, R;Lindorff-Larsen, ...
NATURE COMMUNICATIONS
2019-05-02

2018

From 1,4-Disaccharide to 1,3-Glycosyl Carbasugar: Synthesis of a Bespoke Inhibitor of Family GH99 Endo-alpha-mannosidase
Lu, D(Lu, Dan);Zhu, S(Zhu, Sha);Sobala, LF(Sobala, Lukasz F.);Bernardo-Seisdedos, G(Bernardo-Seisdedos, Ganeko);Milet, O(Milet, Oscar);Zhang, YM(Zhang, Yongmin);Jimenez-Barbero, J(Jimenez-Barbero, ...
ORGANIC LETTERS
2018-12-07
Repurposing ciclopirox as a pharmacological chaperone in a model of congenital erythropoietic porphyria
Urquiza, P(Urquiza, Pedro);Lain, A(Lain, Ana);Sanz-Parra, A(Sanz-Parra, Arantza);Moreno, J(Moreno, Jorge);Bernardo-Seisdedos, G(Bernardo-Seisdedos, Ganeko);Dubus, P(Dubus, Pierre);Gonzalez, ...
SCIENCE TRANSLATIONAL MEDICINE
2018-09-19
Structural basis of pyrrole polymerization in human porphobilinogen deaminase
Pluta, P(Pluta, Paula);Roversi, P(Roversi, Pietro);Bernardo-Seisdedos, G(Bernardo-Seisdedos, Ganeko);Rojas, AL(Rojas, Adriana L.);Cooper, JB(Cooper, Jonathan B.);Gu, S(Gu, Shuang);Pickersgill, ...
BIOCHIMICA ET BIOPHYSICA ACTA
2018-09-01
Structural basis and energy landscape for the Ca2+ gating and calmodulation of the Kv7.2 K+ channel
Bernardo-Seisdedos, G(Bernardo-Seisdedos, Ganeko);Nunez, E(Nunez, Eider);Gomis, C(Gomis, Carolina);Malo, C(Malo, Covadonga);Villarroel, A(Villarroel, Alvaro);Millet, O(Millet, Oscar)
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
2018-03-06

2017

Disulfide driven folding for a conditionally disordered protein
Fraga, H(Fraga, Hugo);Pujols, J(Pujols, Jordi);Gil-Garcia, M(Gil-Garcia, Marcos);Roque, A(Roque, Alicia);Bernardo-Seisdedos, G(Bernardo-Seisdedos, Ganeko);Santambrogio, C(Santambrogio, Carlo);Bech-Serra, ...
SCIENTIFIC REPORTS
2017-12-05
Missense UROS mutations causing congenital erythropoietic porphyria reduce UROS homeostasis that can be rescued by proteasome inhibition
Blouin, JM(Blouin, Jean-Marc);Bernardo-Seisdedos, G(Bernardo-Seisdedos, Ganeko);Sasso, E(Sasso, Emma);Esteve, J(Esteve, Julie);Ged, C(Ged, Cecile);Lalanne, M(Lalanne, Magalie);Sanz-Parra, A(Sanz-Parra, ...
HUMAN MOLECULAR GENETICS
2017-04-15
Contribution of Shape and Charge to the Inhibition of a Family GH99 endo-alpha-1,2-Mannanase
Petricevic, M(Petricevic, Marija);Sobala, LF(Sobala, Lukasz F.);Fernandes, PZ(Fernandes, Pearl Z.);Raich, L(Raich, Lluis);Thompson, AJ(Thompson, Andrew J.);Bernardo-Seisdedos, G(Bernardo-Seisdedos, ...
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
2017-01-25

2014

Pivoting between calmodulin lobes triggered by calcium in the Kv7.2/calmodulin complex.
Alaimo, Alessandro; Alberdi, Araitz; Gomis-Perez, Carolina; Fernandez-Orth, Juncal; Bernardo-Seisdedos, Ganeko; Malo, Covadonga; Millet, Oscar; Areso, Pilar; Villarroel, Alvaro
PLOS ONE
2014-01-01

Protein stability (thermodynamic and kinetic) drives the biophysical properties of the polypeptide chain (protein folding) and the protein's concentration in the cellular environment (protein homeostasis). It is the result of a delicate balance between inter- and intramolecular interactions, which can be easily altered by mutations and/or upon changes in the composition of the surrounding media. In this context, NMR spectroscopy offers a plethora of suitable experiments to investigate protein stability. In our laboratory we are currently interested in the following topics:

  • Pharmacological chaperones. Rare diseases (~7000 identified to date) are an area of significant medical need affecting an estimated 350 million people worldwide, with ~95% having no currently approved drug treatment. They are often produced by inherited mutations affecting the activity of a protein and It is becoming increasily clear that, most frequently, a mutation destabilizes the protein/enzyme, ultimately affecting its intracellular homeostasis. In this context, pharmacological chaperones (small molecules which bind to the protein, restoring stability and activity without affecting its function) can be applied to many diseases. In our laboratory we are investigating new methods (NMR, biophysical and biochemical) for the discovery and characterization of pharmacological chaperones against a set of diseases: congenital eryhtropoietic porphyria, tyrosianemia.
     
  • Environmental modulation of enzyme stability. The high catalytic efficiency and the exquisite enantioselectivity of an enzyme has been employed in some industrial processes to upgrade their properties in order to make them more environmentally-friendly. However, large scale industrial implementation of biotechnological reactions is often limited by the marginal stability of the enzyme in the reactor conditions. In our laboratory we employ NMR and circular dichroism to investigate the effect of external crowding agents to improve the activity and stability of several enzymes. Specifically, we are investigating the mechanism for protein haloadaptation by a combined use of site directed mutagenesis and high-resolution NMR spectroscopy.

Protocolos de Histología

Latest Publications

Multi-Omics Integration Highlights the Role of Ubiquitination in CCl4-Induced Liver Fibrosis

Mercado-Gomez, M;Lopitz-Otsoa, F;Azkargorta, M;Serrano-Maci, M;Lachiondo-Ortega, S;Goikoetxea-Usandizaga, N;Rodriguez-Agudo, R;Fernandez-Ramos, D;Bizkarguenaga, M;Gutierrez-de Juan, V;Lectez, ...

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

2020-12-01

The L-alpha-Lysophosphatidylinositol/G Protein-Coupled Receptor 55 System Induces the Development of Nonalcoholic Steatosis and Steatohepatitis

Fondevila, MF;Fernandez, U;Gonzalez-Rellan, MJ;Lima, ND;Buque, X;Gonzalez-Rodriguez, A;Alonso, C;Iruarrizaga-Lejarreta, M;Delgado, TC;Varela-Rey, M;Senra, A;Garcia-Outeiral, V;Novoa, E;Iglesias, ...

HEPATOLOGY

2020-11-13

S-adenosyl-L-methionine (SAMe) halts the autoimmune response in patients with primary biliary cholangitis (PBC) via antioxidant and S-glutathionylation processes in cholangiocytes

Kilanczyk, E;Banales, JM;Wunsch, E;Barbier, O;Avila, MA;Mato, JM;Milkiewicz, M;Milkiewicz, P

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

2020-11-01

SARS-CoV-2 Infection Dysregulates the Metabolomic and Lipidomic Profiles of Serum

Bruzzone, C;Bizkarguenaga, M;Gil-Redondo, R;Diercks, T;Arana, E;de Vicuna, AG;Seco, M;Bosch, A;Palazon, A;San Juan, I;Lain, A;Gil-Martinez, J;Bernardo-Seisdedos, G;Fernandez-Ramos, D;Lopitz-Otsoa, ...

ISCIENCE

2020-10-23

Lysine and Arginine Protein Post-translational Modifications by Enhanced DIA Libraries: Quantification in Murine Liver Disease

Robinson, AE;Binek, A;Venkatraman, V;Searle, BC;Holewinski, RJ;Rosenberger, G;Parker, SJ;Basisty, N;Xie, XS;Lund, PJ;Saxena, G;Mato, JM;Garcia, BA;Schilling, B;Lu, SC;Van Eyk, JE

JOURNAL OF PROTEOME RESEARCH

2020-10-02

Reciprocal Regulation Between Forkhead Box M1/NF-kappa B and Methionine Adenosyltransferase 1A Drives Liver Cancer

Li, Y;Lu, LQ;Tu, J;Zhang, J;Xiong, T;Fan, W;Wang, JH;Li, M;Chen, YB;Steggerda, J;Peng, H;Chen, YH;Li, TWH;Zhou, ZG;Mato, JM;Seki, E;Liu, T;Yang, HP;Lu, SC

HEPATOLOGY

2020-10-01

Arachidyl amido cholanoic acid improves liver glucose and lipid homeostasis in nonalcoholic steatohepatitisviaAMPK and mTOR regulation

Fernandez-Ramos, D;Lopitz-Otsoa, F;Delacruz-Villar, L;Bilbao, J;Pagano, M;Mosca, L;Bizkarguenaga, M;Serrano-Macia, M;Azkargorta, M;Iruarrizaga-Lejarreta, M;Sot, J;Tsvirkun, D;van Liempd, SM;Goni, ...

WORLD JOURNAL OF GASTROENTEROLOGY

2020-09-14

Abnormal concentration of porphyrins in serum from COVID-19 patients

San Juan, I;Bruzzone, C;Bizkarguenaga, M;Bernardo-Seisdedos, G;Lain, A;Gil-Redondo, R;Diercks, T;Gil-Martinez, J;Urquiza, P;Arana, E;Seco, M;de Vicuna, AG;Embade, N;Mato, JM;Millet, O

BRITISH JOURNAL OF HAEMATOLOGY

2020-08-29

PINE: An Automation Tool to Extract and Visualize Protein-Centric Functional Networks

Sundararaman, N;Go, J;Robinson, AE;Mato, JM;Lu, SC;Van Eyk, JE;Venkatraman, V

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

2020-07-01

H-1 NMR-Based Urine Metabolomics Reveals Signs of Enhanced Carbon and Nitrogen Recycling in Prostate Cancer

Bruzzone, C;Loizaga-Iriarte, A;Sanchez-Mosquera, P;Gil-Redondo, R;Astobiza, I;Diercks, T;Cortazar, AR;Ugalde-Olano, A;Schafer, H;Blanco, FJ;Unda, M;Cannet, C;Spraul, M;Mato, JM;Embade, N;Carracedo, ...

JOURNAL OF PROTEOME RESEARCH

2020-06-05