Ana Maria Gimeno Cardells
agimeno
Ana Maria Gimeno Cardells
EMERGING SCIENTIST
Ramón y Cajal (RyC) Programme Ikerbasque Research Fellow
Ramón y Cajal (RyC) Programme Ikerbasque Research Fellow
Chemical Glycobiology Lab
Address: Bizkaia Science and Technology Park, building 800, Derio (Bizkaia)

Ana Gimeno obtained her BSc in Chemistry from the University of Valencia (Spain) and continued her scientific pursuits by completing her PhD in Chemistry from the same University in 2014.

In 2015, Dr. Gimeno joined the Chemical Glycobiology Lab at CIC bioGUNE as a postdoctoral researcher. During a 5-years period, she worked in the molecular recognition field, and especially in the applications of NMR to study the binding of glycans to relevant human lectins and antibodies. In 2020, Dr. Gimeno moved to Utrecht University, joining the laboratory of Prof. GJ Boons, to widen her scientific expertise, focusing on bioconjugation methodologies and chemo-enzymatic synthesis of glycoproteins.

In 2023, Dr. Gimeno joined CIC bioGUNE as a Ramón y Cajal and Ikerbasque Research Fellow (tenure track positions) in the Chemical Glycobiology Lab. Since 2024, Dr. Gimeno currently holds the position of Emerging Scientist. Her research is focused on developing chemical and chemical biology tools to address socially relevant biomedical questions. Currently, she exploits different approaches, combining NMR with molecular biology and different biophysical techniques, trying to discern how glycosylation changes impact on cancer progression at the chemical and molecular level.

Her contributions span across the fields of chemistry and biology, helping to unveil the structural, dynamic and energetic details in the binding of glycans to human lectins and antibodies using NMR as the primary tool. She also applies multidisciplinary approaches to disentangle the 3D structural features of complex oligosaccharides and glycoconjugates, as well as to prepare and discern the molecular fingerprint of protein conjugates, including synthetic glycoproteins with therapeutic potential. Over the years she has established an extensive network of collaborations world-wide, in the field of Glycobiology, and in Supramolecular Chemistry and Drug Discovery, with the main objective to determine the structural basis of molecular recognition processes.

Awards & recognitions
Ikerbasque Research Fellow (2023). Ramon y Cajal Research Fellow (2023). Juan de la Cierva Fellow (2015). Graduate Research Fellow-FPI programme, Valencian Community (2009). BSc Excellence Award in Chemistry, University of Valencia (2008).

Collaborations
X. Huang (Michigan State University), R. Kikkeri (Indian Institute of Science Education and Research), F. Marcelo (New university of Lisbon), F. García (University of La Rioja), N. Martín (IMDEA Nanociencia & Complutense University, Madrid).

Links
She is co-leader at CIC bioGUNE of the doctoral network Glyco-N, “GLYCOprotein N-glycosylation from non-life to eukaryotes”, started on March 2024 (cordis.europa.eu/project/id/101119499)

Latest Publications

2022

2021

2020

The Chemical Glycobiology Lab is actively engaged in extensive multidisciplinary studies on diverse glycan-lectin systems related to health and disease. This collaborative research involves Senior (Jimenez-Barbero, Ardá, Ereño-Orbea, Unione) and Emerging Scientists (Gimeno), along with Specialists, Technicians, Postdoctoral associates, and Doctoral candidates.

Using a combination of diverse and synergic methodologies, including chemical synthesis, protein biochemistry and molecular biology, biophysics (ITC, BLI), molecular modelling, and structural biology (X-Ray crystallography and CryoEM), with special emphasis on NMR spectroscopy, the Chemical Glycobiology lab investigates the interactions of glycans related to immune responses, from cancer to bacterial and viral infections.

Indeed, glycans, prevalent on mammalian cells and in the extracellular matrix, collectively form the glycocalyx, impacting physical properties, regulating protein function, and acting as ligands for lectins. Dysregulation of glycosyltransferases results in tumor-associated carbohydrate antigens (TACAs), unique to tumor cells. While TACAs are recognized therapeutic targets, their low immunogenicity presents a challenge for T-cell-dependent immune responses crucial in cancer therapy. TACAs play a crucial role in modulating immune responses within the tumor microenvironment through interactions with lectins. Similar interactions are observed in bacterial and viral-mediated infectious events.

From the application side, the focus is on structure-based drug and vaccine design, collaborating with research groups and pharmaceutical companies worldwide, investigating lectin-glycan and antibody-glycan molecular recognition processes. Additionally, from the basic perspective, we explore the conformational properties of glycans, specially the glycosyl oxocarbenium cations, key intermediates in synthesis and cleavage, and examine the presentation and dynamic properties of glycans designed for specific geometrical shapes.

In parallel, the laboratory continuously develops cutting-edge NMR-based techniques. Special emphasis is placed on the use of stable isotopes (13C, 19F) to push the boundaries of NMR applications. These advancements are vital for understanding the pivotal roles of glycans in their interactions with receptors, influencing biological responses associated with health or disease.

Specific areas and research lines include:

  • • Interactions of glycans with galectins and C-type lectins, overseen by Dr. Ana Ardá.
  • • Recognition of sialic acids by Siglecs and their role as glyco-immune checkpoints, led by Dr. June Ereño-Orbea.
  • • Attachment of glycans to influenza virus proteins and other pathogens, investigating their impact on infections, directed by Dr. Luca Unione.
  • • Analysis of proteoglycans and the functional landscape of glycosylation in cancer, spearheaded by Dr. Ana Gimeno.
Latest Publications

Antigenic determinants driving serogroup-specific antibody response to Neisseria meningitidis C, W, and Y capsular polysaccharides: Insights for rational vaccine design

Pietri, GP; Bertuzzi, S; Karnicar, K; Unione, L; Lisnic, B; Malic, S; Miklic, K; Novak, M; Calloni, I; Santini, L; Usenik, A; Romano, MR; Adamo, R; Jonjic, S; Turk, D; Jimenez-Barbero, J; Rovis, ...

CARBOHYDRATE POLYMERS

2024-10-01

Disentangling Glycan-Protein Interactions: Nuclear Magnetic Resonance (NMR) to the Rescue.

Bertuzzi, Sara; Poveda, Ana; Arda, Ana; Gimeno, Ana; Jimenez-Barbero, Jesus;

Journal of visualized experiments : JoVE

2024-05-17

A Multidisciplinary Structural Approach to the Identification of the Haemophilus influenzae Type b Capsular Polysaccharide Protective Epitope

Nonne, F; Dello Iacono, L; Bertuzzi, S; Unione, L; Proietti, D; Norais, N; Margarit, I; Adamo, R; Jiménez-Barbero, J; Carboni, F; Romano, MR;

ACS CENTRAL SCIENCE

2024-02-22

Dissecting the Conformational Stability of a Glycan Hairpin

Yadav, N; Djalali, S; Poveda, A; Ricardo, MG; Seeberger, PH; Jiménez-Barbero, J; Delbianco, M;

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

2024-02-20

Unraveling Molecular Recognition of Glycan Ligands by Siglec-9 via NMR Spectroscopy and Molecular Dynamics Modeling

Atxabal, U; Nycholat, C; Pröpster, JM; Fernández, A; Oyenarte, I; Lenza, MP; Franconetti, A; Soares, CO; Coelho, H; Marcelo, F; Schubert, M; Paulson, JC; Jiménez-Barbero, J; Ereño-Orbea, J;

ACS CHEMICAL BIOLOGY

2024-02-16

Evolution of human H3N2 influenza virus receptor specificity has substantially expanded the receptor-binding domain site

Thompson, AJ; Wu, NC; Canales, A; Kikuchi, C; Zhu, XY; de Toro, BF; Cañada, FJ; Worth, C; Wang, SY; Mcbride, R; Peng, WJ; Nycholat, CM; Jiménez-Barbero, J; Wilson, IA; Paulson, JC;

CELL HOST & MICROBE

2024-02-14

Strategy for the Enzymatic Acylation of the Apple Flavonoid Phloretin Based on Prior α-Glucosylation

Gonzalez-Alfonso, JL; Alonso, C; Poveda, A; Ubiparip, Z; Ballesteros, AO; Desmet, T; Jiménez-Barbero, J; Coderch, L; Plou, FJ;

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

2024-02-13

The impact of glycosylation on the structure, function, and interactions of CD14.

Quintana, Jon Imanol; Delgado, Sandra; Rabano, Miriam; Azkargorta, Mikel; Florencio-Zabaleta, Mirane; Unione, Luca; Vivanco, Maria dM; Elortza, Felix; Jimenez-Barbero, Jesus; Arda, Ana;

Glycobiology

2024-01-16

Illuminating a Solvent-Dependent Hierarchy for Aromatic CH/π Complexes with Dynamic Covalent Glyco-Balances

Díaz-Casado, L; Villacampa, A; Corzana, F; Jiménez-Barbero, J; Gómez, AM; Santana, AG; Asensio, JL;

JACS AU

2024-01-02

Targeting the Spike: Repurposing Mithramycin and Dihydroergotamine to Block SARS-CoV-2 Infection

Stagnoli, S; Macari, G; Corsi, P; Capone, B; Vidaurrazaga, A; Ereño-Orbea, J; Ardá, A; Polticelli, F; Jiménez-Barbero, J; Abrescia, NGA; Coluzza, I;

ACS OMEGA

2023-11-13

Synthesis of a glycan hairpin

Fittolani, G; Tyrikos-Ergas, T; Poveda, A; Yu, Y; Yadav, N; Seeberger, PH; Jiménez-Barbero, J; Delbianco, M;

NATURE CHEMISTRY

2023-07-03

Total Synthesis and Structural Studies of Zwitterionic Bacteroides fragilis Polysaccharide A1 Fragments

Wang, Z; Poveda, A; Zhang, QJ; Unione, L; Overkleeft, HS; van der Marel, GA; Jesús, JB; Codée, JDC;

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

2023-06-13

Structural insights into Siglec-15 reveal glycosylation dependency for its interaction with T cells through integrin CD11b

Lenza, MP; Egia-Mendikute, L; Antoñana-Vildosola, A; Soares, CO; Coelho, H; Corzana, F; Bosch, A; Manisha, P; Quintana, JI; Oyenarte, I; Unione, L; Moure, MJ; Azkargorta, M; Atxabal, U; Sobczak, ...

NATURE COMMUNICATIONS

2023-06-13

KR-12 Derivatives Endow Nanocellulose with Antibacterial and Anti-Inflammatory Properties: Role of Conjugation Chemistry

Blasi-Romero, A; Angström, M; Franconetti, A; Muhammad, T; Jiménez-Barbero, J; Göransson, U; Palo-Nieto, C; Ferraz, N;

ACS APPLIED MATERIALS & INTERFACES

2023-05-11

Blood pH Analysis in Combination with Molecular Medical Tools in Relation to COVID-19 Symptoms

Siebert, HC; Eckert, T; Bhunia, A; Klatte, N; Mohri, M; Siebert, S; Kozarova, A; Hudson, JW; Zhang, RY; Zhang, N; Li, L; Gousias, K; Kanakis, D; Yan, MD; Jiménez-Barbero, J; Kozár, T; Nifantiev, ...

BIOMEDICINES

2023-05-11

Molecular Recognition of Glycan-Bearing Glycomacromolecules Presented at Membrane Surfaces by Lectins: An NMR View

Lete, MG; Hoffmann, M; Schomann, N; Martínez-Castillo, A; Peccati, F; Konietzny, PB; Delgado, S; Snyder, NL; Jiménez-Oses, G; Abrescia, NGA; Ardá, A; Hartmann, L; Jiménez-Barbero, J;

ACS OMEGA

2023-05-03

SARS-CoV-2 Spike N-Terminal Domain Engages 9-O-Acetylated?2-8-Linked Sialic Acids

Tomris, I; Unione, L; Nguyen, L; Zaree, P; Bouwman, KM; Liu, L; Li, ZS; Fok, JA; Carrasco, MR; van der Woude, R; Kimpel, ALM; Linthorst, MW; Kilavuzoglu, SE; Verpalen, ECJM; Caniels, TG; Sanders, ...

ACS CHEMICAL BIOLOGY

2023-04-27

Highly Diastereoselective Multicomponent Synthesis of Spirocyclopropyl Oxindoles Enabled by Rare-Earth Metal Salts

Tallarida, MA; Olivito, F; Navo, CD; Algieri, V; Jiritano, A; Costanzo, P; Poveda, A; Moure, MJ; Jiménez-Barbero, J; Maiuolo, L; Jiménez-Osés, G; De Nino, A;

ORGANIC LETTERS

2023-04-26

Development of synthetic, self-adjuvanting, and self-assembling anticancer vaccines based on a minimal saponin adjuvant and the tumor-associated MUC1 antigen

Pifferi, C; Aguinagalde, L; Ruiz-de-Angulo, A; Sacristán, N; Baschirotto, PT; Poveda, A; Jiménez-Barbero, J; Anguita, J; Fernández-Tejada, A;

CHEMICAL SCIENCE

2023-03-01

Biomimetic Tweezers for N-Glycans: Selective Recognition of the Core GlcNAc2 Disaccharide of the Sialylglycopeptide SGP

Milanesi, F; Unione, L; Ardá, A; Nativi, C; Jiménez-Barbero, J; Roelens, S; Francesconi, O;

CHEMISTRY-A EUROPEAN JOURNAL

2023-02-24