Our team explores the structure and dynamics of biological molecules by cryoEM. The technique allows observing large complexes at atomic resolution and can separate different conformational states. These features open up the possibility to characterize the conformational landscape of the sample of interest while performing its function. In essence, we can examine several intermediates of a particular biological process in just “one shot” and reveal sequential steps of the reaction in detail. We work on several biological systems:
Research line 1: Large oligomeric enzymes
One line of research involves observing enzymes like Pyruvate Carboxylase and Glutamate Dehydrogenase during catalysis using cryoEM to elucidate their enzymatic mechanisms and allosteric regulations.
Research line 2: Structure of flexible filamentous plant viruses
This line of research focuses on the structure of flexible filamentous plant viruses, including the first atomic structure delivered for a potyvirus and demonstrating structural similarities between potexviruses and potyviruses.
Research line 3: Ribosomal structures
The third line focuses on ribosomal structures elucidating mechanisms of nascent protein folding and translation regulation.
Collaborations
Our group has a network of national and international collaborations in each line of research: Liang Tong (Columbia University, USA) and Natalia Lisa (IBR, CONICET-UNR, Argentina) on enzymatic oligomers. Miguel Aranda (CEBAS-CSIC, Murcia) and Fernando Ponz (CBGP, Madrid) on flexible filamentous plant viruses. Marina Rodnina (Max Planck Institute for Biophysical Chemistry, Germany) on ribosomal complexes.
Links
https://www.nature.com/articles/s41467-022-33987-2
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Group Leader
Mikel Valle
TECHNICIANS / DOCTORAL CANDIDATES -
Aileen Santini Santiago
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Fernando Lamas Lopez
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Melisa Lázaro
Members
Latest Publications
Understanding the key features of the spontaneous formation of bona fide prions through a novel methodology that enables their swift and consistent generation
Eraña, H; Díaz-Domínguez, CM; Charco, JM; Vidal, E; González-Miranda, E; Pérez-Castro, MA; Piñeiro, P; López-Moreno, R; Sampedro-Torres-Quevedo, C; Fernández-Veiga, L; Tasis-Galarza, J; Lorenzo, ...
ACTA NEUROPATHOLOGICA COMMUNICATIONS
2023-09-07
Genomic insights into metabolic flux in hummingbirds
Gershman, A; Hauck, Q; Dick, M; Jamison, JM; Tassia, M; Agirrezabala, X; Muhammad, S; Ali, R; Workman, RE; Valle, M; Wong, GW; Welch, KC Jr; Timp, W;
GENOME RESEARCH
2023-05-01
Effect of ATG12-ATG5-ATG16L1 autophagy E3-like complex on the ability of LC3/GABARAP proteins to induce vesicle tethering and fusion
Iriondo, MN; Etxaniz, A; Varela, YR; Ballesteros, U; Lázaro, M; Valle, M; Fracchiolla, D; Martens, S; Montes, LR; Goñi, FM; Alonso, A;
CELLULAR AND MOLECULAR LIFE SCIENCES
2023-02-01
CryoEM structural exploration of catalytically active enzyme pyruvate carboxylase
López-Alonso, JP; Lázaro, M; Gil-Cartón, D; Choi, PH; Tong, L; Valle, M;
NATURE COMMUNICATIONS
2022-10-19
Autophagy protein LC3C binding to phospholipid and interaction with lipid membranes
Ballesteros, U; Etxaniz, A; Iriondo, MN; Varela, YR; Lazaro, M; Viguera, AR; Montes, LR; Valle, M; Goni, FM; Alonso, A;
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
2022-05-26
A switch from α-helical to β-strand conformation during co-translational protein folding
Agirrezabala, X; Samatova, E; Macher, M; Liutkute, M; Maiti, M; Gil-Carton, D; Novacek, J; Valle, M; Rodnina, M;
EMBO JOURNAL
2022-01-07
3D architecture and structural flexibility revealed in the subfamily of large glutamate dehydrogenases by a mycobacterial enzyme
Lázaro, M; Melero, R; Huet, C; López-Alonso, JP; Delgado, S; Dodu, A; Bruch, EM; Abriata, LA; Alzari, PM; Valle, M; Lisa, MN;
COMMUNICATIONS BIOLOGY
2021-06-03
Structural Characterization of N-Linked Glycans in the Receptor Binding Domain of the SARS-CoV-2 Spike Protein and their Interactions with Human Lectins
Lenza, MP; Oyenarte, I; Diercks, T; Quintana, JI; Gimeno, A; Coelho, H; Diniz, A; Peccati, F; Delgado, S; Bosch, A; Valle, M; Millet, O; Abrescia, NGA; Palazn, A; Marcelo, F; Jiménez-Osés, G; ...
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
2020-10-22
Photoacoustic effect applied on model membranes and living cells: direct observation with multiphoton excitation microscopy and long-term viability analysis
Galisteo-González, F; Monasterio, BG; Gil, D; Valle, M; Goñi, FM;
SCIENTIFIC REPORTS
2020-01-15
Structure of Turnip mosaic virus and its viral-like particles
Cuesta, R; Yuste-Calvo, C; Gil-Cartón, D; Sánchez, F; Ponz, F; Valle, M;
SCIENTIFIC REPORTS
2019-10-28
Insights into real-time chemical processes in a calcium sensor protein-directed dynamic library
Canal-Martín, A; Sastre, J; Sánchez-Barrena, MJ; Canales, A; Baldominos, S; Pascual, N; Martínez-González, L; Molero, D; Fernández-Valle, ME; Sáez, E; Blanco-Gabella, P; Gómez-Rubio, E; Martín-Santamaría, ...
NATURE COMMUNICATIONS
2019-06-26
Structural Homology Between Nucleoproteins of ssRNA Viruses
Valle, M; Harris Jr; Bhella, D;
VIRUS PROTEIN AND NUCLEOPROTEIN COMPLEXES
2018-01-01
Potyvirus virion structure shows conserved protein fold and RNA binding site in ssRNA viruses
Zamora, M; Méndez-López, E; Agirrezabala, X; Cuesta, R; Lavín, JL; Sánchez-Pina, MA; Aranda, MA; Valle, M;
SCIENCE ADVANCES
2017-09-01
Does Ceramide Form Channels? The Ceramide-Induced Membrane Permeabilization Mechanism
Artetxe, I; Ugarte-Uribe, B; Gil, D; Valle, M; Alonso, A; García-Sáez, AJ; Goñi, FM;
BIOPHYSICAL JOURNAL
2017-08-22
Coating Graphene Oxide with Lipid Bilayers Greatly Decreases Its Hemolytic Properties
Monasterio, BG; Alonso, B; Sot, J; García-Arribas, AB; Gil-Cartón, D; Valle, M; Zurutuza, A; Goñi, FM;
LANGMUIR
2017-08-22
Ribosome rearrangements at the onset of translational bypassing
Agirrezabala, X; Samatova, E; Klimova, M; Zamora, M; Gil-Carton, D; Rodnina, MV; Valle, M;
SCIENCE ADVANCES
2017-06-01
"Pyruvate Carboxylase, Structure and Function"
Valle, M; Harris Jr; MarlesWright, J;
MACROMOLECULAR PROTEIN COMPLEXES: STRUCTURE AND FUNCTION
2017-01-01
Identification of a Membrane-bound Prepore Species Clarifies the Lytic Mechanism of Actinoporins
Morante, K; Bellomio, A; Gil-Cartón, D; Redondo-Morata, L; Sot, J; Scheuring, S; Valle, M; González-Mañas, JM; Tsumoto, K; Caaveiro, JMM;
JOURNAL OF BIOLOGICAL CHEMISTRY
2016-09-09
Lipid Modulation of LC3/GABARAP-Mediated Autophagosomal Elongation
Alonso, A; Landajuela, A; Hervas, JH; Anton, Z; Montes, LR; Gil, D; Valle, M; Rodriguez, JF; Goni, FM;
BIOPHYSICAL JOURNAL
2016-02-16
Lipid Geometry and Bilayer Curvature Modulate LC3/GABARAP-Mediated Model Autophagosomal Elongation
Landajuela, A; Hervás, JH; Antón, Z; Montes, LR; Gil, D; Valle, M; Rodriguez, JF; Goñi, FM; Alonso, A;
BIOPHYSICAL JOURNAL
2016-01-19