0000-0002-9663-0669Rosa Barrio started her work on ubiquitin-like genes during her PhD at the Center of Molecular Biology Severo Ochoa (Madrid, Spain), where she characterized the ubiquitin genes in Drosophila. During her postdoctoral stay in the laboratory of Prof FC Kafatos (Harvard University-USA, IMBB-Greece, EMBL-Germany) she initiated her work on SALL transcription factors, which she continued at the laboratory of Dr JF de Celis and Prof García-Bellido at the CBMSO (Madrid, Spain). Mutations in the genes encoding those factors cause hereditary human diseases, such as the Townes-Brocks and the Okihiro syndromes (TBS and OS). She obtained a Ramón y Cajal contract in 2003. Since December 2004 she is in charge of the Laboratory 1 at the Functional Genomics Unit of CIC bioGUNE. The laboratory is currently investigating different aspects of the role of SALL and SUMO during development. In addition, the laboratory developed new technology for the isolation of proteins modified by SUMO and other Ubiquitin-likes, both in vivo and in cultured cells, either in Drosophila or in mammalian systems, which will have wide applications in the field. Rosa Barrio coordinated two European projects, one of them dedicated to the training of early stage researchers (ITN program, UPStream); the other grant being a large consortium of European groups interested in the field of the ubiquitin-likes (COST, PROTEOSTASIS).
Latest Publications
2022
2021
2020
Post-translational modifications by ubiquitin-like modifiers (proteins similar to ubiquitin) can influence many aspects of protein homeostasis, such are stability, localization or activity. SUMOylation is a reversible process by which SUMO, the Small Ubiquitin-like Modifier, is attached to target proteins and modify their properties. We discovered that SUMO has an important role in cholesterol intake, contributing to steroid hormone synthesis through the regulation of the Scavenger Receptors SR-B1 and the nuclear receptor Ftz-f1, homologous to the human Steroidogenic Factor 1, SF-1. The regulation of hormonal synthesis is a crucial step that determines animal viability and size. This novel function of SUMOylation is conserved in several tissues in different organisms. To investigate more in detail the function of the ubiquitin-likes, we are developing new molecular tools to isolate and identify proteins modified by SUMO and other Ubiquitin-like modifiers in human cells and in model systems, such is Drosophila. We generated a versatile, highly specific, easy-to-use toolbox composed of vectors useful to analyze the modification of a protein of interest or to identify modified sub-proteomes. Among the transcription factors regulated by SUMOylation, we are interested on the SPALT-Like (SALL) family of proteins, necessary for numerous biological processes. Mutations in SALL1 and SALL4 cause the rare diseases Townes-Brocks and Okihiro (Duane-Radial Ray) Syndromes, respectively, being also involved in the susceptibility to tumors. Patients might present dysplastic kidneys, supernumerary thumbs, malformed ears, sensorineural hearing loss and severe growth retardation. We are investigating the mechanism by which a truncated form of SALL1 causes the Townes-Brocks symptoms. We hypothesized that these are caused by the malfunctioning of primary cilia and we are currently performing the experiments necessary to prove this hypothesis. Primary fibroblasts derived from a Townes-Brocks patient, which exhibit mutation in the gene SALL1. The fibroblasts were kindly provided by Dr. Wilkie, Oxford, UK. Markers: Acetylated tubulin (red), F-actin (green), DNA (blue). Representation of SUMO evolution across species, with associated functional consequences in human, Blattella and Drosophila.
VISIT OUR LAB WEBPAGE for more information
-
Associate Principal Investigator
James D Sutherland
POSTDOCTORAL RESEARCHER -
Orhi Barroso Gomila
TECHNICIANS / DOCTORAL CANDIDATES -
Claudia Guinea Perez
-
Verónica Muratore
-
Laura Merino Cacho
-
Mónica Pozo Rodríguez
-
Coralia Pérez Fernández
Members
Latest Publications
Structural insights into Siglec-15 reveal glycosylation dependency for its interaction with T cells through integrin CD11b
Lenza, MP; Egia-Mendikute, L; Antoñana-Vildosola, A; Soares, CO; Coelho, H; Corzana, F; Bosch, A; Manisha, P; Quintana, JI; Oyenarte, I; Unione, L; Moure, MJ; Azkargorta, M; Atxabal, U; Sobczak, ...
NATURE COMMUNICATIONS
2023-06-13
Studying the ubiquitin code through biotin-based labelling methods
Barroso-Gomila, O; Muratore, V; Merino-Cacho, L; Rodriguez, JA; Barrio, R; Sutherland, JD;
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
2022-11-16
In memoriam: Huib Ovaa, the brain behind the formula (18/12/1973-19/05/2020)
Barrio, R; Rodriguez, MS;
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
2022-11-16
U1A is a positive regulator of the expression of heterologous and cellular genes involved in cell proliferation and migration
Rovira, E; Moreno, B; Razquin, N; Hjerpe, R; Gonzalez-Lopez, M; Barrio, R; de los Mozos, IR; Ule, J; Pastor, F; Blazquez, L; Fortes, P;
MOLECULAR THERAPY-NUCLEIC ACIDS
2022-05-25
Editorial: 17th Spanish Society for Developmental Biology Meeting: New Trends in Developmental Biology
Araujo, SJ; Barrio, R;
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
2022-04-20
Origin and Development of the Adipose Tissue, a Key Organ in Physiology and Disease
Parra-Peralbo, E; Talamillo, A; Barrio, R;
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
2021-12-21
A Genotyped Case of Townes-Brocks Syndrome with Absent Pulmonary Valve Syndrome from Turkey
Ilhan, O; Gumus, E; Hakan, N; Istar, H; Harmandar, B; Olgun, H; Karakus, SC; Cullu, N; Kohlhase, J; Sutherland, JD; Barrio, R;
JOURNAL OF PEDIATRIC GENETICS
2021-12-10
Identification of proximal SUMO-dependent interactors using SUMO-ID
Barroso-Gomila, O; Trulsson, F; Muratore, V; Canosa, I; Merino-Cacho, L; Cortazar, AR; Pérez, C; Azkargorta, M; Iloro, I; Carracedo, A; Aransay, AM; Elortza, F; Mayor, U; Vertegaal, ACO; Barrio, ...
NATURE COMMUNICATIONS
2021-11-18
NEDDYLATION INHIBITION REDUCES LIVER STEATOSIS IN MAFLD MICE MODELS BY PROMOTING HEPATIC FATTY ACID OXIDATION VIA DEPTOR-mTOR AXIS
Serrano-Maciá, M; Espinosa, JS; González-Rellan, MJ; Azkargorta, M; Goikoetxea-Usandizaga, N; Lopitz-Otsoa, F; De Urturi, DS; Agudo, RR; Lachiondo-Ortega, S; Mercado-Gómez, M; Bizkarguenaga, ...
HEPATOLOGY
2021-10-01
SALL1 Modulates CBX4 Stability, Nuclear Bodies, and Regulation of Target Genes
Giordano, I; Pirone, L; Muratore, V; Landaluze, E; Pérez, C; Lang, V; Garde-Lapido, E; Gonzalez-Lopez, M; Barroso-Gomila, O; Vertegaal, ACO; Aransay, AM; Rodriguez, JA; Rodriguez, MS; Sutherland, ...
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
2021-09-21
Neddylation inhibition ameliorates steatosis in NAFLD by boosting hepatic fatty acid oxidation via the DEPTOR-mTOR axis
Serrano-Maciá, M; Simón, J; González-Rellan, MJ; Azkargorta, M; Goikoetxea-Usandizaga, N; Lopitz-Otsoa, F; De Urturi, DS; Rodríguez-Agudo, R; Lachiondo-Ortega, S; Mercado-Gomez, M; de Juan, ...
MOLECULAR METABOLISM
2021-07-13
LUZP1 Controls Cell Division, Migration and Invasion Through Regulation of the Actin Cytoskeleton
Bozal-Basterra, L; Gonzalez-Santamarta, M; Muratore, V; Martín-Martín, N; Ercilla, A; Rodríguez, JA; Carracedo, A; Sutherland, JD; Barrio, R;
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
2021-04-01
Virtual meeting, real and sound science: report of the 17th Meeting of the Spanish Society for Developmental Biology (SEBD-2020) Virtual Meeting. 18th to 20th November 2020 .
Araújo, SJ; Almudi, I; Bozal-Basterra, L; Casares, F; Casas-Tintó, S; Escalante, A; Garcia-Moreno, F; Losada-Pérez, M; Maeso, I; Marcon, L; Ocaña, OH; Pampliega, O; Rada-Iglesias, A; Rayon, ...
INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY
2021-01-01
Multi-Omics Integration Highlights the Role of Ubiquitination in CCl4-Induced Liver Fibrosis
Mercado-Gómez, M; Lopitz-Otsoa, F; Azkargorta, M; Serrano-Maci, M; Lachiondo-Ortega, S; Goikoetxea-Usandizaga, N; Rodríguez-Agudo, R; Fernández-Ramos, D; Bizkarguenaga, M; Gutiérrez-de Juan, ...
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
2020-12-01
HuR/ELAVL1 drives malignant peripheral nerve sheath tumor growth and metastasis
Palomo-Irigoyen, M; Pérez-Andrés, E; Iruarrizaga-Lejarreta, M; Barreira-Manrique, A; Tamayo-Caro, M; Vila-Vecilla, L; Moreno-Cugnon, L; Beitia, N; Medrano, D; Fernández-Ramos, D; Lozano, JJ; ...
JOURNAL OF CLINICAL INVESTIGATION
2020-07-01
LUZP1, a novel regulator of primary cilia and the actin cytoskeleton, is a contributing factor in Townes-Brocks Syndrome
Bozal-Basterra, L; Gonzalez-Santamarta, M; Muratore, V; Bermejo-Arteagabeitia, A; Da Fonseca, C; Barroso-Gomila, O; Azkargorta, M; Iloro, I; Pampliega, O; Andrade, R; Martín-Martín, N; Branon, ...
ELIFE
2020-06-18
Genetic manipulation of LKB1 elicits lethal metastatic prostate cancer
Hermanova, I; Zúñiga-García, P; Caro-Maldonado, A; Fernandez-Ruiz, S; Salvador, F; Martín-Martín, N; Zabala-Letona, A; Nuñez-Olle, M; Torrano, V; Camacho, L; Lizcano, JM; Talamillo, A; Carreira, ...
JOURNAL OF EXPERIMENTAL MEDICINE
2020-06-01
SUMOylation in the control of cholesterol homeostasis
Talamillo, A; Ajuria, L; Grillo, M; Barroso-Gomila, O; Mayor, U; Barrio, R;
OPEN BIOLOGY
2020-05-06
The role of SUMOylation during development
Talamillo, A; Barroso-Gomila, O; Giordano, I; Ajuria, L; Grillo, M; Mayor, U; Barrio, R;
BIOCHEMICAL SOCIETY TRANSACTIONS
2020-04-01
Targeting PML in triple negative breast cancer elicits growth suppression and senescence
Arreal, L; Piva, M; Fernandez, S; Revandkar, A; Clerigué, AS; Villanueva, J; Zabala-Letona, A; Pujana, M; Astobiza, I; Cortazar, AR; Hermanova, I; Bozal-Basterra, L; Arruabarrena-Aristorena, ...
CELL DEATH AND DIFFERENTIATION
2020-04-01