Félix Royo
froyo
Félix Royo
POST DOCTORAL RESEARCHER
Exosomes Lab
Email: froyo.ciberehd@cicbiogune.es
Phone: 4310
Address: Bizkaia Science and Technology Park, building 800, Derio (Bizkaia)
Latest Publications
Extracellular vesicles in hepatology: Physiological role,involvement in pathogenesis, and therapeutic opportunities
Azparren-Angulo, M;Royo, F;Gonzalez, E;Liebana, M;Brotons, B;Berganza, J;Goni-de-Cerio, F;Manicardi, N;Abad-Jorda, L;Gracia-Sancho, J;Falcon-Perez, JM
PHARMACOLOGY & THERAPEUTICS
2021-02-01
Extracellular Vesicles From Liver Progenitor Cells Downregulates Fibroblast Metabolic Activity and Increase the Expression of Immune-Response Related Molecules
Royo, F;Azkargorta, M;Lavin, JL;Clos-Garcia, M;Cortazar, AR;Gonzalez-Lopez, M;Barcena, L;del Portillo, HA;Yanez-Mo, M;Marcilla, A;Borras, FE;Peinado, H;Guerrero, I;Vales-Gomez, M;Cereijo, U;Sardon, ...
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
2021-01-12

2020

Methods for Separation and Characterization of Extracellular Vesicles: Results of a Worldwide Survey Performed by the ISEV Rigor and Standardization Subcommittee
Royo, F;Thery, C;Falcon-Perez, JM;Nieuwland, R;Witwer, KW
CELLS
2020-09-01

2019

Metabolic Nano-Machines: Extracellular Vesicles Containing Active Enzymes and Their Contribution to Liver Diseases
Angulo, MA (Azparren Angulo, Maria)[ 1 ] ; Royo, F (Royo, Felix)¡; Falcón-Pérez, JM (Falcon-Perez, Juan M.)
CURRENT PATHOBIOLOGY REPORTS
2019-12-01
Assessing the role of surface glycans of extracellular vesicles on cellular uptake
Williams, C;Pazos, R;Royo, F;Gonzalez, E;Roura-Ferrer, M;Martinez, A;Gamiz, J;Reichardt, NC;Falcon-Perez, JM
SCIENTIFIC REPORTS
2019-08-15
Gut microbiome and serum metabolome analyses identify molecular biomarkers and altered glutamate metabolism in fibromyalgia
Clos-Garcia, M;Andres-Marin, N;Fernandez-Eulate, G;Abecia, L;Lavin, JL;van Liempd, S;Cabrera, D;Royo, F;Valero, A;Errazquin, N;Vega, MCG;Govillard, L;Tackett, MR;Tejada, G;Gonzalez, E;Anguita, ...
EBIOMEDICINE
2019-08-01
Tetraspanin-decorated extracellular vesicle-mimetics as a novel adaptable reference material
Lozano-Andres, E;Libregts, SF;Toribio, V;Royo, F;Morales, S;Lopez-Martin, S;Vales-Gomez, M;Reyburn, HT;Falcon-Perez, JM;Wauben, MH;Soto, M;Yanez-Mo, M
JOURNAL OF EXTRACELLULAR VESICLES
2019-06-01
Modification of the glycosylation of extracellular vesicles alters their biodistribution in mice
Royo, F;Cossio, U;de Angulo, AR;Llop, J;Falcon-Perez, JM
NANOSCALE
2019-01-28
Raman tweezers microspectroscopy of circa 100 nm extracellular vesicles
Kruglik, SG;Royo, F;Guigner, JM;Palomo, L;Seksek, O;Turpin, PY;Tatischeff, I;Falcon-Perez, JM
NANOSCALE
2019-01-28
Differences in the metabolite composition and mechanical properties of extracellular vesicles secreted by hepatic cellular models
Royo, F;Gil-Carton, D;Gonzalez, E;Mleczko, J;Palomo, L;Perez-Cormenzana, M;Mayo, R;Alonso, C;Falcon-Perez, JM
JOURNAL OF EXTRACELLULAR VESICLES
2019-01-01
A Comprehensive Study of Vesicular and Non-Vesicular miRNAs from a Volume of Cerebrospinal Fluid Compatible with Clinical Practice
Prieto-Fernandez, E;Aransay, AM;Royo, F;Gonzalez, E;Lozano, JJ;Santos-Zorrozua, B;Macias-Camara, N;Gonzalez, M;Garay, RP;Benito, J;Garcia-Orad, A;Falcon-Perez, JM
THERANOSTICS
2019-01-01

2018

Abundance of Cytochromes in Hepatic Extracellular Vesicles Is Altered by Drugs Related With Drug-Induced Liver Injury
Palomo, L(Palomo, Laura);Mleczko, JE(Mleczko, Justyna Emilia);Azkargorta, M(Azkargorta, Mikel);Conde-Vancells, J(Conde-Vancells, Javier);Gonzalez, E(Gonzalez, Esperanza);Elortza, F(Elortza, ...
HEPATOLOGY COMMUNICATIONS
2018-09-01
PLAGL1 gene function during hepatoma cells proliferation.
Vega-Benedetti AF; Saucedo CN; Zavattari P; Vanni R; Royo F; Llavero F; Zugaza JL; Parada LA;.
ONCOTARGET
2018-08-28
Characterization of Plasmodium vivax Proteins in Plasma-Derived Exosomes From Malaria-Infected Liver-Chimeric Humanized Mice
Gualdron-Lopez, M(Gualdron-Lopez, Melisa);Flannery, EL(Flannery, Erika L.);Kangwanrangsan, N(Kangwanrangsan, Niwat);Chuenchob, V(Chuenchob, Vorada);Fernandez-Orth, D(Fernandez-Orth, Dietmar);Segui-Barber, ...
FRONTIERS IN MICROBIOLOGY
2018-06-25
Metabolic alterations in urine extracellular vesicles are associated to prostate cancer pathogenesis and progression
Clos-Garcia, M(Clos-Garcia, Marc);Loizaga-Iriarte, A(Loizaga-Iriarte, Ana);Zuniga-Garcia, P(Zuniga-Garcia, Patricia);Sanchez-Mosquera, P(Sanchez-Mosquera, Pilar);Cortazar, AR(Rosa Cortazar, ...
JOURNAL OF EXTRACELLULAR VESICLES
2018-05-07
Glycosylation of extracellular vesicles: current knowledge, tools and clinical perspectives
Williams, C(Williams, Charles);Royo, F(Royo, Felix);Aizpurua-Olaizola, O(Aizpurua-Olaizola, Oier);Pazos, R(Pazos, Raquel);Boons, GJ(Boons, Geert-Jan);Reichardt, NC(Reichardt, Niels-Christian);Falcon-Perez, ...
JOURNAL OF EXTRACELLULAR VESICLES
2018-03-04

2017

A novel community driven software for functional enrichment analysis of extracellular vesicles data
Pathan, M(Pathan, Mohashin);Keerthikumar, S(Keerthikumar, Shivakumar);Chisanga, D(Chisanga, David);Alessandro, R(Alessandro, Riccardo);Ang, CS(Ang, Ching-Seng);Askenase, P(Askenase, Philip);Batagov, ...
JOURNAL OF EXTRACELLULAR VESICLES
2017-05-26
Hepatocyte-secreted extracellular vesicles modify blood metabolome and endothelial function by an arginase-dependent mechanism
Royo, F(Royo, Felix);Moreno, L(Moreno, Laura);Mleczko, J(Mleczko, Justyna);Palomo, L(Palomo, Laura);Gonzalez, E(Gonzalez, Esperanza);Cabrera, D(Cabrera, Diana);Cogolludo, A(Cogolludo, Angel);Vizcaino, ...
SCIENTIFIC REPORTS
2017-02-17
Metabolically active extracellular vesicles released from hepatocytes under drug-induced liver-damaging conditions modify serum metabolome and might affect different pathophysiological processes
Royo, F(Royo, Felix);Palomo, L(Palomo, Laura);Mleczko, J(Mleczko, Justyna);Gonzalez, E(Gonzalez, Esperanza);Alonso, C(Alonso, Cristina);Martinez, I(Martinez, Ibon);Perez-Cormenzana, M(Perez-Cormenzana, ...
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES : OFFICIAL JOURNAL OF THE EUROPEAN FEDERATION FOR PHARMACEUTICAL SCIENCES
2017-02-15

2016

Comparative miRNA Analysis of Urine Extracellular Vesicles Isolated through Five Different Methods
Royo, F(Royo, Felix);Diwan, I(Diwan, Izzuddin);Tackett, MR(Tackett, Michael R.);Zuniga, P(Zuniga, Patricia);Sanchez-Mosquera, P(Sanchez-Mosquera, Pilar);Loizaga-Iriarte, A(Loizaga-Iriarte, Ana);Ugalde-Olano, ...
CANCERS
2016-12-01
Vesicle-MaNiA: extracellular vesicles in liquid biopsy and cancer
Torrano, V(Torrano, Veronica);Royo, F(Royo, Felix);Peinado, H(Peinado, Hector);Loizaga-Iriarte, A(Loizaga-Iriarte, Ana);Unda, M(Unda, Miguel);Falcon-Perez, JM(Falcon-Perez, Juan M.);Carracedo, ...
CURRENT OPINION IN PHARMACOLOGY
2016-08-01
Transcriptomic profiling of urine extracellular vesicles reveals alterations of CDH3 in prostate cancer
Royo, F(Royo, Felix);Zuniga-Garcia, P(Zuniga-Garcia, Patricia);Torrano, V(Torrano, Veronica);Loizaga, A(Loizaga, Ana);Sanchez-Mosquera, P(Sanchez-Mosquera, Pilar);Ugalde-Olano, A(Ugalde-Olano, ...
ONCOTARGET
2016-02-09
Different EV enrichment methods suitable for clinical settings yield different subpopulations of urinary extracellular vesicles from human samples
Royo, F(Royo, Felix);Zuniga-Garcia, P(Zuniga-Garcia, Patricia);Sanchez-Mosquera, P(Sanchez-Mosquera, Pilar);Egia, A(Egia, Ainara);Perez, A(Perez, Amparo);Loizaga, A(Loizaga, Ana);Arceo, R(Arceo, ...
JOURNAL OF EXTRACELLULAR VESICLES
2016-01-01

2015

Circulating RNA: looking at the liver through a frosted glass
Falcon-Perez, JM(Falcon-Perez, J. M.);Royo, F(Royo, F.)
BIOMARKERS : BIOCHEMICAL INDICATORS OF EXPOSURE, RESPONSE, AND SUSCEPTIBILITY TO CHEMICALS
2015-10-03
Methodological aspects of the molecular and histological study of prostate cancer: Focus on PTEN
Ugalde-Olano, A(Ugalde-Olano, Aitziber);Egia, A(Egia, Ainara);Fernandez-Ruiz, S(Fernandez-Ruiz, Sonia);Loizaga-Iriarte, A(Loizaga-Iriarte, Ana);Zuniga-Garcia, P(Zuniga-Garcia, Patricia);Garcia, ...
METHODS
2015-05-01

2014

Microarray-Based Identification of Lectins for the Purification of Human Urinary Extracellular Vesicles Directly from Urine Samples
Echevarria, Juan; Royo, Felix; Pazos, Raquel; Salazar, Lorena; Manuel Falcon-Perez, Juan; Reichardt, Niels-Christian
CHEMBIOCHEM
2014-07-21
Quantitative proteomic analysis of hepatocyte-secreted extracellular vesicles reveals candidate markers for liver toxicity.
Rodriguez-Suarez, Eva; Gonzalez, Esperanza; Hughes, Chris; Conde-Vancells, Javier; Rudella, Andrea; Royo, Felix; Palomo, Laura; Elortza, Felix; Lu, Shelly C.; Mato, Jose M.; Vissers, Johannes ...
JOURNAL OF PROTEOMICS
2014-05-30
A Pilot Study on the Potential of RNA-Associated to Urinary Vesicles as a Suitable Non-Invasive Source for Diagnostic Purposes in Bladder Cancer.
Perez, Amparo; Loizaga, Ana; Arceo, Raquel; Lacasa, Isabel; Rabade, Ainara; Zorroza, Kerman; Mosen-Ansorena, David; Gonzalez, Esperanza; Aransay, Ana M.; Falcon-Perez, Juan M.; Unda-Urzaiz, ...
CANCERS
2014-01-22
Human mammospheres secrete hormone-regulated active extracellular vesicles.
Gonzalez, Esperanza; Piva, Marco; Rodriguez-Suarez, Eva; Gil, David; Royo, Felix; Elortza, Felix; Falcon-Perez, Juan M.; Vivanco, Maria Dm
PLOS ONE
2014-01-01
Considerations for applying metabolomics to the analysis of extracellular vesicles.
Palomo, Laura; Casal, Enriqueta; Royo, Felix; Cabrera, Diana; van-Liempd, Sebastiaan; Falcon-Perez, Juan M.
FRONTIERS IN IMMUNOLOGY
2014-01-01
Sorting signal targeting mRNA into hepatic extracellular vesicles.
Szostak, Natalia; Royo, Felix; Rybarczyk, Agnieszka; Szachniuk, Marta; Blazewicz, Jacek; del Sol, Antonio; Falcon-Perez, Juan M.
RNA BIOLOGY
2014-01-01

2013

Transcriptome of Extracellular Vesicles Released by Hepatocytes
Royo, Felix; Schlangen, Karin; Palomo, Laura; Gonzalez, Esperanza; Conde-Vancells, Javier; Berisa, Agustin; Aransay, Ana M.; Falcon-Perez, Juan M.
PLOS ONE
2013-07-11
Combined Fluorescent-Chromogenic In Situ Hybridization for Identification and Laser Microdissection of Interphase Chromosomes
Paz, Nerea; Zabala, Amaia; Royo, Felix; Garcia-Orad, Africa; Zugaza, Jose L.; Parada, Luis A.
PLOS ONE
2013-04-02

2012

Proteomic analysis of microvesicles from plasma of healthy donors reveals high individual variability
Bastos-Amador, Patricia; Royo, Felix; Gonzalez, Esperanza; Conde-Vancells, Javier; Palomo-Diez, Laura; Borras, Francesc E.; Falcon-Perez, Juan M.
JOURNAL OF PROTEOMICS
2012-06-27
Proteostasis of tau. Tau overexpression results in its secretion via membrane vesicles
Simon, Diana; Garcia-Garcia, Esther; Royo, Felix; Manuel Falcon-Perez, Juan; Avila, Jesus
FEBS LETTERS
2012-01-02

2010

Functional blockade of alpha(5)beta(1) integrin induces scattering and genomic landscape remodeling of hepatic progenitor cells
Vellon, Luciano; Royo, Felix; Matthiesen, Rune; Torres-Fuenzalida, Jose; Lorenti, Alicia; Parada, Luis A.
BMC CELL BIOLOGY
2010-10-19

2009

Spatial link between nucleoli and expression of the Zac1 gene
Royo, Felix; Paz, Nerea; Espinosa, Luis; McQueen, Philip G.; Vellon, Luciano; Parada, Luis A.
CHROMOSOMA
2009-12-01
Concentration compared with total urinary excretion of 11,17-DOA in cynomolgus monkey urine
Hau, J.; Royo, F.
JOURNAL OF MEDICAL PRIMATOLOGY
2009-08-01

The discovery of cell-secreted extracellular vesicles (EVs) including EXOSOMES has provided a new cellular component with the ability to influence different biological and pathological processes. In the last years these vesicles has attracted the interest in clinical, pharmaceutical and manufacturing areas. All cellular systems in culture or forming part of a tissue in the body secrete EVs containing proteins, nucleic acids, lipids and metabolites into the environment. They have been shown to act as important mediators of intercellular communication and regulators of cellular niches, and their altered characteristics in many diseases suggest them to be helpful for the diagnostic purposes. Our group has been working in exosomes since 2006 and acquired wide experience in isolate and characterize these vesicles from different biofluids and cell lines, in normal and pathological conditions. Our current scientific interests are:

1) To elucidate the functional role of exosomes in metabolism.

The goal is to identify genes, proteins and metabolites that form part of the exosomes in normal and pathological conditions. The improvement in the knowledge of the cargo of these vesicles will provide also clues about the functional role and implications of them in biology. Technological advances in the past 20 years have permitted large-scale measurements of biochemical and cellular constituents for study as a unified whole, creating all the ‘-omics’ technologies including proteomics, genomics and more recently metabolomics.  The group has already showed the presence in hepatic exosomes of an elevated number of metabolic enzymes involved in endogenous and xenobiotics compounds, and we are currently studying the implications of these vesicles in the metabolism of those compounds.

2) To develop diagnosis and therapeutics tools based on exosomes.

Exosomes constitutes a platform to identify low-invasive disease biomarkers. The group is currently comparing the composition of exosomes in different scenarios in order to generate a repertoire of differentially expressed molecules that could be candidate biomarker of disease. In addition, behind the idea to obtain a "magic bullet" – a desired activity encapsulated in a vehicle with a known specific target- the group is trying to define the cellular preferences displayed by exosomes. In this aspect the group is focused in identifying the molecular determinants that define the cellular specificity, and also the molecular machinery involved in the biogenesis of exosomes. A better knowledge of the biology and the mechanisms of action of exosomes will help to manipulate these vesicles with therapeutics purposes.

Latest Publications

Downregulation of Glutamine Synthetase, not glutaminolysis, is responsible for glutamine addiction in Notch1-driven acute lymphoblastic leukemia

Nguyen, TL;Nokin, MJ;Teres, S;Tome, M;Bodineau, C;Galmar, O;Pasquet, JM;Rousseau, B;van Liempd, S;Falcon-Perez, JM;Richard, E;Muzotte, E;Rezvani, HR;Priault, M;Bouchecareilh, M;Redonnet-Vernhet, ...

MOLECULAR ONCOLOGY

2021-02-13

Extracellular vesicles in hepatology: Physiological role,involvement in pathogenesis, and therapeutic opportunities

Azparren-Angulo, M;Royo, F;Gonzalez, E;Liebana, M;Brotons, B;Berganza, J;Goni-de-Cerio, F;Manicardi, N;Abad-Jorda, L;Gracia-Sancho, J;Falcon-Perez, JM

PHARMACOLOGY & THERAPEUTICS

2021-02-01

Identification of Isomeric N-Glycans by Conformer Distribution Fingerprinting using Ion Mobility Mass Spectrometry

Torano, JS;Aizpurua-Olaizola, O;Wei, N;Li, TH;Unione, L;Jimenez-Oses, G;Corzana, F;Somovilla, VJ;Falcon-Perez, JM;Boons, GJ

CHEMISTRY-A EUROPEAN JOURNAL

2021-01-14

Extracellular Vesicles From Liver Progenitor Cells Downregulates Fibroblast Metabolic Activity and Increase the Expression of Immune-Response Related Molecules

Royo, F;Azkargorta, M;Lavin, JL;Clos-Garcia, M;Cortazar, AR;Gonzalez-Lopez, M;Barcena, L;del Portillo, HA;Yanez-Mo, M;Marcilla, A;Borras, FE;Peinado, H;Guerrero, I;Vales-Gomez, M;Cereijo, U;Sardon, ...

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY

2021-01-12

Borrelia burgdorferi infection induces long-term memory-like responses in macrophages with tissue-wide consequences in the heart

Barriales, D;Martin-Ruiz, I;Carreras-Gonzalez, A;Montesinos-Robledo, M;Azkargorta, M;Iloro, I;Escobes, I;Martin-Mateos, T;Atondo, E;Palacios, A;Gonzalez-Lopez, M;Barcena, L;Cortazar, AR;Cabrera, ...

PLOS BIOLOGY

2021-01-01

3D Cell Cultures as Prospective Models to Study Extracellular Vesicles in Cancer

Bordanaba-Florit, G;Madarieta, I;Olalde, B;Falcon-Perez, JM;Royo, F

CANCERS

2021-01-01

International Society for Extracellular Vesicles and International Society for Cell and Gene Therapy statement on extracellular vesicles from mesenchymal stromal cells and other cells: considerations for potential therapeutic agents to suppress coronavirus disease-19

Borger, V;Weiss, DJ;Anderson, JD;Borras, FE;Bussolati, B;Carter, DRF;Dominici, M;Falcon-Perez, JM;Gimona, M;Hill, AF;Hoffman, AM;de Kleijn, D;Levine, BL;Lim, R;Lotvall, J;Mitsialis, SA;Monguio-Tortajada, ...

CYTOTHERAPY

2020-09-01

Methods for Separation and Characterization of Extracellular Vesicles: Results of a Worldwide Survey Performed by the ISEV Rigor and Standardization Subcommittee

Royo, F;Thery, C;Falcon-Perez, JM;Nieuwland, R;Witwer, KW

CELLS

2020-09-01

Variability in Cerebrospinal Fluid MicroRNAs Through Life

Prieto-Fernandez, E;Lopez-Lopez, E;Martin-Guerrero, I;Barcena, L;Gonzalez-Lopez, M;Aransay, AM;Lozano, JJ;Benito, J;Falcon-Perez, JM;Garcia-Orad, A

MOLECULAR NEUROBIOLOGY

2020-07-16

Integrative Analysis of Fecal Metagenomics and Metabolomics in Colorectal Cancer

Clos-Garcia, M;Garcia, K;Alonso, C;Iruarrizaga-Lejarreta, M;D'Amato, M;Crespo, A;Iglesias, A;Cubiella, J;Bujanda, L;Falcon-Perez, JM

CANCERS

2020-05-01