Carlos Miguel Diaz Dominguez
cdiaz
Carlos Miguel Diaz Dominguez
POSTDOCTORAL RESEARCHER
Prion Research Lab
Address: Bizkaia Science and Technology Park, building 502, Derio (Bizkaia)

Transmissible spongiform encephalopathies (TSEs) are lethal neurodegenerative diseases caused by prions, abnormal forms of a physiological protein that induce disease by inducing their aberrant conformation to the native counterpart. Understanding how prions cross species barriers and cause disease is crucial for developing diagnostic tools and treatments. The Prion Research Lab focuses on unraveling these mechanisms and has made significant strides in sensitive prion detection, validating the "protein-only" hypothesis, and developing methods to produce recombinant prions in vitro. Their work provides vital insights into prion diseases and informs efforts to combat them effectively.

Research line 1: Study of the molecular basis underlying prion diseases
To unveil the molecular mechanisms underlying prion pathogenic conversion, we investigate, among others, polyanionic cofactors' role in facilitating this spontaneous process. We aim to design and utilize tailored cofactors to elucidate how the innocuous PrP protein transforms into a self-replicating, disease-causing form, occurring in 85 % of diagnosed cases. Additionally, we produce diverse recombinant prion proteins to study their infectious capabilities, identifying sequences conducive or resistant to misfolding. Structural studies using cryo-electron microscopy on these recombinant prion strains aim to decipher how distinct structural features correlate with their biological characteristics in animal models.

Research line 2: Development of new approaches for the treatment and diagnosis of prion diseases
Our central goal involves developing methods to detect prions in urine and other easily accessible samples as disease biomarkers for individuals with prion-related mutations. Simultaneously, we are exploring new therapeutic avenues by evaluating potential anti-prion compounds and developing gene therapy strategies using dominant negative proteins to inhibit prion propagation.

Research line 3: Research on other neurodegenerative disorders related to protein misfolding
Our research extends to other neurodegenerative disorders associated with protein misfolding. Recent discoveries revealing shared molecular mechanisms between prion diseases and conditions like Parkinson’s disease, synucleinopathies, Alzheimer’s disease, and Frontotemporal Dementia have prompted our group to apply tools initially developed for prion research to these disorders. Specifically, techniques for detecting misfolded proteins are being adapted for diagnostic purposes across these conditions.

Collaborations
Dr. Castilla’s group collaborates with over 20 international and national research teams to advance scientific boundaries. Internationally, they partner with esteemed institutions such as the Prion Research Centre at the University of Colorado, the Cell Biology Department at Boston University, and the Neuropathology Department at the University Hospital in Zurich. Nationally, they collaborate with institutions including IRTA-CReSA in Barcelona, the University of Salamanca, CiMUS Research Centre at the University of Santiago de Compostela, and the Veterinary School at the University of Zaragoza. They also work with hospitals such as Hospital Clínic de Barcelona, Marqués de Valdecilla in Santander, and leading Basque health research institutes BioDonostia, BioAraba, and BioBizkaia. Additionally, Dr. Castilla closely engages with the Spanish Foundation for Prion Diseases to understand patient and family needs.

Links
Spanish Foundation for Prion Diseases: https://fundacionprionicas.org/

Latest Publications

Classical BSE dismissed as the cause of CWD in Norwegian red deer despite strain similarities between both prion agents

Marín-Moreno, A; Benestad, SL; Barrio, T; Pirisinu, L; Espinosa, JC; Tran, L; Huor, A; Di Bari, MA; Eraña, H; Maddison, B; DAgostino, C; Fernández-Borges, N; Canoyra, S; Jerez-Garrido, N; Castilla, ...

VETERINARY RESEARCH

2024-05-15

A Protein Misfolding Shaking Amplification-based method for the spontaneous generation of hundreds of bona fide prions

Erana, H; Sampedro-Torres-Quevedo, C; Charco, JM; Diaz-Dominguez, CM; Peccati, F; San-Juan-Ansoleaga, M; Vidal, E; Goncalves-Anjo, N; Perez-Castro, MA; Gonzalez-Miranda, E; Pineiro, P; Fernandez-Veiga, ...

NATURE COMMUNICATIONS

2024-03-08

A tetracationic porphyrin with dual anti-prion activity

Masone, A; Zucchelli, C; Caruso, E; Lavigna, G; Eraña, H; Giachin, G; Tapella, L; Comerio, L; Restelli, E; Raimondi, I; Elezgarai, SR; De Leo, F; Quilici, G; Taiarol, L; Oldrati, M; Lorenzo, ...

ISCIENCE

2023-09-15

Understanding the key features of the spontaneous formation of bona fide prions through a novel methodology that enables their swift and consistent generation

Eraña, H; Díaz-Domínguez, CM; Charco, JM; Vidal, E; González-Miranda, E; Pérez-Castro, MA; Piñeiro, P; López-Moreno, R; Sampedro-Torres-Quevedo, C; Fernández-Veiga, L; Tasis-Galarza, J; Lorenzo, ...

ACTA NEUROPATHOLOGICA COMMUNICATIONS

2023-09-07

Analysis of a large case series of fatal familial insomnia to determine tests with the highest diagnostic value

Kortazar-Zubizarreta, I; Eraña, H; Pereda, A; Charco, JM; Manero-Azua, A; Ruiz-Onandi, R; Aguirre, U; Gonzalez-Chinchon, G; de Nanclares, GP; Castilla, J; Basque Prionopathies Study Grp;

JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY

2023-01-20

An optimized Western blot assay provides a comprehensive assessment of the physiological endoproteolytic processing of the prion protein

Vanni, I; Iacobone, F; DAgostino, C; Giovannelli, M; Pirisinu, L; Altmeppen, HC; Castilla, J; Torres, JM; Agrimi, U; Nonno, R;

JOURNAL OF BIOLOGICAL CHEMISTRY

2023-01-18

Bona fide atypical scrapie faithfully reproduced for the first time in a rodent model

Vidal, E; Sánchez-Martín, MA; Eraña, H; Lázaro, SP; Pérez-Castro, MA; Otero, A; Charco, JM; Marín, B; López-Moreno, R; Díaz-Domínguez, CM; Geijo, M; Ordóñez, M; Cantero, G; di Bari, M; Lorenzo, ...

ACTA NEUROPATHOLOGICA COMMUNICATIONS

2022-12-13

Glycans are not necessary to maintain the pathobiological features of bovine spongiform encephalopathy

Otero, A; Barrio, T; Eraña, H; Charco, JM; Betancor, M; Díaz-Domínguez, CM; Marín, B; Andréoletti, O; Torres, JM; Kong, QZ; Badiola, JJ; Bolea, R; Castilla, J;

PLOS PATHOGENS

2022-10-01

Description of the first Spanish case of Gerstmann-Straussler-Scheinker disease with A117V variant: clinical, histopathological and biochemical characterization

Eraña, H; San Millán, B; Díaz-Domínguez, CM; Charco, JM; Rodríguez, R; Viéitez, I; Pereda, A; Yañez, R; Geijo, M; Navarro, C; de Nanclares, GP; Teijeira, S; Castilla, J;

JOURNAL OF NEUROLOGY

2022-03-16

Homozygous R136S mutation in PRNP gene causes inherited early onset prion disease

Ximelis, T; Marín-Moreno, A; Espinosa, JC; Eraña, H; Charco, JM; Hernández, I; Riveira, C; Alcolea, D; González-Roca, E; Aldecoa, I; Molina-Porcel, L; Parchi, P; Rossi, M; Castilla, J; Ruiz-García, ...

ALZHEIMERS RESEARCH & THERAPY

2021-10-18

Biosemiotics comprehension of PrP code and prion disease

Coca, JR; Eraña, H; Castilla, J;

BIOSYSTEMS

2021-09-10

Cerebrospinal Fluid and Plasma Small Extracellular Vesicles and miRNAs as Biomarkers for Prion Diseases

López-Pérez, O; Sanz-Rubio, D; Hernaiz, A; Betancor, M; Otero, A; Castilla, J; Andréoletti, O; Badiola, JJ; Zaragoza, P; Bolea, R; Toivonen, JM; Martín-Burriel, I;

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

2021-07-01

Sporadic Creutzfeldt-Jakob disease with extremely long 14-year survival period

Kortazar-Zubizarreta, I; Ruiz-Onandi, R; Pereda, A; Vado, Y; González-Chinchon, G; Eraña, H; de Nanclares, GP; Castilla, J;

EUROPEAN JOURNAL OF NEUROLOGY

2021-06-26

Improving the Pharmacological Properties of Ciclopirox for Its Use in Congenital Erythropoietic Porphyria

Bernardo-Seisdedos, G; Charco, JM; SanJuan, I; García-Martínez, S; Urquiza, P; Eraña, H; Castilla, J; Millet, O;

JOURNAL OF PERSONALIZED MEDICINE

2021-06-01

Detection of chronic wasting disease in mule and white-tailed deer by RT-QuIC analysis of outer ear

Ferreira, NC; Charco, JM; Plagenz, J; Orru, CD; Denkers, ND; Metrick, MA; Hughson, AG; Griffin, KA; Race, B; Hoover, EA; Castilla, J; Nichols, TA; Miller, MW; Caughey, B;

SCIENTIFIC REPORTS

2021-04-08

Prion-Associated Neurodegeneration Causes Both Endoplasmic Reticulum Stress and Proteasome Impairment in a Murine Model of Spontaneous Disease

Otero, A; Betancor, M; Eraña, H; Borges, NF; Lucas, JJ; Badiola, JJ; Castilla, J; Bolea, R;

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

2021-01-01

A Novel, Reliable and Highly Versatile Method to Evaluate Different Prion Decontamination Procedures

Eraña, H; Pérez-Castro, MA; García-Martínez, S; Charco, JM; López-Moreno, R; Díaz-Dominguez, CM; Barrio, T; González-Miranda, E; Castilla, J;

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY

2020-10-29

Autoantibodies against the prion protein in individuals with PRNP mutations

Frontzek, K; Carta, M; Losa, M; Epskamp, M; Meisl, G; Anane, A; Brandel, JP; Camenisch, U; Castilla, J; Haïk, S; Knowles, T; Lindner, E; Lutterotti, A; Minikel, EV; Roiter, I; Safar, JG; Sanchez-Valle, ...

NEUROLOGY

2020-10-06

Evaluation of the Influence of Astrocytes on In Vitro Blood-Brain Barrier Models

García-Salvador, A; Domínguez-Monedero, A; Gómez-Fernández, P; García-Bilbao, A; Carregal-Romero, S; Castilla, J; Goñi-de-Cerio, F;

ATLA-ALTERNATIVES TO LABORATORY ANIMALS

2020-07-01

Detection of Pathognomonic Biomarker PrPSc and the Contribution of Cell Free-Amplification Techniques to the Diagnosis of Prion Diseases

Eraña, H; Charco, JM; González-Miranda, E; García-Martínez, S; López-Moreno, R; Pérez-Castro, MA; Díaz-Domínguez, CM; García-Salvador, A; Castilla, J;

BIOMOLECULES

2020-03-01