Andreas Schedlbauer
Andreas Schedlbauer
POST DOCTORAL RESEARCHER
Ribosome Structural Biology Lab
Address: Bizkaia Science and Technology Park, building 800, Derio (Bizkaia)
Latest Publications

2016

2015

2013

Amino acids are assembled into proteins following the genetic instructions encoded in the mRNA in a process called translation. This is primarily governed by a large macromolecular machine called the ribosome. Research in the Fucini and Connell labs focuses on understanding, at a biochemical and structural level, the molecular details of processes that regulate the ribosome at several levels including:

1) Inhibition of core ribosomal activities by antibiotics. Medically relevant antibiotics target the ribosome and inhibit its core functions to exert their anti-microbial activities. We aim to understand how these antibiotics interact with and inhibit the ribosome to improve existing drugs or develop novel antibiotics.
2) Regulation of core activities like initiation, elongation and termination by native protein factors. During protein synthesis the ribosome has several functional activates which are coordinated by specific protein factors. We aim to understand how these factors work together with the ribosome.
3) Ribosome Biosynthesis: Assembling a ribosome requires orchestrating the structural integration of more than 55 ribosomal proteins and three nucleic acid strands in Escherichia coli. This assembly process is facilitated by ribosome assembly factors and we aim to understand how these factors guide the maturation of the ribosome.
4) Co-translational folding and protein sorting. The nascent protein is synthesized in the core of the ribosome and passes through a 100Å long conduit to emerge outside the ribosome. This passage is a highly dynamic and 'personalized' event, where the ribosome and the protein chain communicate to regulate the translation and compartmentalization of the nascent protein. We aim to understand the details of this communication.

Accordingly, the groups have expertise in the preparation and structural characterization of ribosomal complexes. To provide a complete structural understanding of ribosomal functions we employ complementary structural biology methods like X-ray crystallography, NMR (Fucini Lab) and cryo-EM (Connell Lab).

Latest Publications

Draft Genome Sequence of Streptomyces sp. Strain AM-2504, Identified by 16S rRNA Comparative Analysis as a Streptomyces kasugaensis Strain

Napolioni, V;Cimarelli, L;Miano, A;La Teana, A;Capuni, R;Giuliodori, AM;Fabbretti, A;Spurio, R

MICROBIOLOGY RESOURCE ANNOUNCEMENTS

2019-09-01

Characterization of the Self-Resistance Mechanism to Dityromycin in the Streptomyces Producer Strain

Fabbretti, A;Capuni, R;Giuliodori, AM;Cimarelli, L;Miano, A;Napolioni, V;La Teana, A;Spurio, R

MSPHERE

2019-09-01

Binding of the protein ICln to alpha-integrin contributes to the activation of IClswell current

Schedlbauer, A;Tammaz, G;Rodighiero, S;Civello, DA;Tamplenizza, M;Ledolter, K;Nofziger, C;Patsch, W;Konrat, R;Paulmichl, M;Dossena, S

SCIENTIFIC REPORTS

2019-08-21

RsgA couples the maturation state of the 30S ribosomal decoding center to activation of its GTPase pocket

Lopez-Alonso, JP(Lopez-Alonso, Jorge Pedro);Kaminishi, T(Kaminishi, Tatsuya);Kikuchi, T(Kikuchi, Takeshi);Hirata, Y(Hirata, Yuya);Iturrioz, I(Iturrioz, Idoia);Dhimole, N(Dhimole, Neha);Schedlbauer, ...

Nucleic acids research

2017-06-20

Structure of a 30S pre-initiation complex stalled by GE81112 reveals structural parallels in bacterial and eukaryotic protein synthesis initiation pathways

Lopez-Alonso, JP(Lopez-Alonso, Jorge P.);Fabbretti, A(Fabbretti, Attilio);Kaminishi, T(Kaminishi, Tatsuya);Iturrioz, I(Iturrioz, Idoia);Brandi, L(Brandi, Letizia);Gil-Carton, D(Gil-Carton, David);Gualerzi, ...

Nucleic acids research

2017-02-28

The Novel Aminomethylcycline Omadacycline Has High Specificity for the Primary Tetracycline-Binding Site on the Bacterial Ribosome

Heidrich, CG(Heidrich, Corina G.);Mitova, S(Mitova, Sanya);Schedlbauer, A(Schedlbauer, Andreas);Connell, SR(Connell, Sean R.);Fucini, P(Fucini, Paola);Steenbergen, JN(Steenbergen, Judith N.);Berens, ...

Antibiotics (Basel, Switzerland)

2016-12-01

Crystallographic characterization of the ribosomal binding site and molecular mechanism of action of Hygromycin A

Kaminishi, T(Kaminishi, Tatsuya);Schedlbauer, A(Schedlbauer, Andreas);Fabbretti, A(Fabbretti, Attilio);Brandi, L(Brandi, Letizia);Ochoa-Lizarralde, B(Ochoa-Lizarralde, Borja);He, CG(He, Cheng-Guang);Milon, ...

Nucleic acids research

2015-11-16

Structural Characterization of an Alternative Mode of Tigecycline Binding to the Bacterial Ribosome

Schedlbauer, A(Schedlbauer, Andreas);Kaminishi, T(Kaminishi, Tatsuya);Ochoa-Lizarralde, B(Ochoa-Lizarralde, Borja);Dhimole, N(Dhimole, Neha);Zhou, S(Zhou, Shu);Lopez-Alonso, JP(Lopez-Alonso, ...

Antimicrobial agents and chemotherapy

2015-05-01

Solid-state NMR enhanced by dynamic nuclear polarization as a novel tool for ribosome structural biology

Gelis, Ioannis; Vitzthum, Veronika; Dhimole, Neha; Caporini, Marc A.; Schedlbauer, Andreas; Carnevale, Diego; Connell, Sean R.; Fucini, Paola; Bodenhausen, Geoffrey

journal of biomolecular nmr

2013-06-01

Latest Publications

Draft Genome Sequence of Streptomyces sp. Strain AM-2504, Identified by 16S rRNA Comparative Analysis as a Streptomyces kasugaensis Strain

Napolioni, V;Cimarelli, L;Miano, A;La Teana, A;Capuni, R;Giuliodori, AM;Fabbretti, A;Spurio, R

MICROBIOLOGY RESOURCE ANNOUNCEMENTS

2019-09-01

Characterization of the Self-Resistance Mechanism to Dityromycin in the Streptomyces Producer Strain

Fabbretti, A;Capuni, R;Giuliodori, AM;Cimarelli, L;Miano, A;Napolioni, V;La Teana, A;Spurio, R

MSPHERE

2019-09-01

Binding of the protein ICln to alpha-integrin contributes to the activation of IClswell current

Schedlbauer, A;Tammaz, G;Rodighiero, S;Civello, DA;Tamplenizza, M;Ledolter, K;Nofziger, C;Patsch, W;Konrat, R;Paulmichl, M;Dossena, S

SCIENTIFIC REPORTS

2019-08-21

RsgA couples the maturation state of the 30S ribosomal decoding center to activation of its GTPase pocket

Lopez-Alonso, JP(Lopez-Alonso, Jorge Pedro);Kaminishi, T(Kaminishi, Tatsuya);Kikuchi, T(Kikuchi, Takeshi);Hirata, Y(Hirata, Yuya);Iturrioz, I(Iturrioz, Idoia);Dhimole, N(Dhimole, Neha);Schedlbauer, ...

Nucleic acids research

2017-06-20

Structure of a 30S pre-initiation complex stalled by GE81112 reveals structural parallels in bacterial and eukaryotic protein synthesis initiation pathways

Lopez-Alonso, JP(Lopez-Alonso, Jorge P.);Fabbretti, A(Fabbretti, Attilio);Kaminishi, T(Kaminishi, Tatsuya);Iturrioz, I(Iturrioz, Idoia);Brandi, L(Brandi, Letizia);Gil-Carton, D(Gil-Carton, David);Gualerzi, ...

Nucleic acids research

2017-02-28

The Novel Aminomethylcycline Omadacycline Has High Specificity for the Primary Tetracycline-Binding Site on the Bacterial Ribosome

Heidrich, CG(Heidrich, Corina G.);Mitova, S(Mitova, Sanya);Schedlbauer, A(Schedlbauer, Andreas);Connell, SR(Connell, Sean R.);Fucini, P(Fucini, Paola);Steenbergen, JN(Steenbergen, Judith N.);Berens, ...

Antibiotics (Basel, Switzerland)

2016-12-01

Crystallographic characterization of the ribosomal binding site and molecular mechanism of action of Hygromycin A

Kaminishi, T(Kaminishi, Tatsuya);Schedlbauer, A(Schedlbauer, Andreas);Fabbretti, A(Fabbretti, Attilio);Brandi, L(Brandi, Letizia);Ochoa-Lizarralde, B(Ochoa-Lizarralde, Borja);He, CG(He, Cheng-Guang);Milon, ...

Nucleic acids research

2015-11-16

Structural Characterization of an Alternative Mode of Tigecycline Binding to the Bacterial Ribosome

Schedlbauer, A(Schedlbauer, Andreas);Kaminishi, T(Kaminishi, Tatsuya);Ochoa-Lizarralde, B(Ochoa-Lizarralde, Borja);Dhimole, N(Dhimole, Neha);Zhou, S(Zhou, Shu);Lopez-Alonso, JP(Lopez-Alonso, ...

Antimicrobial agents and chemotherapy

2015-05-01

Solid-state NMR enhanced by dynamic nuclear polarization as a novel tool for ribosome structural biology

Gelis, Ioannis; Vitzthum, Veronika; Dhimole, Neha; Caporini, Marc A.; Schedlbauer, Andreas; Carnevale, Diego; Connell, Sean R.; Fucini, Paola; Bodenhausen, Geoffrey

journal of biomolecular nmr

2013-06-01