2010/03/24

Advances in protein quantitation using iTRAQ labeling

Proteomics Platform group at CIC bioGUNE has recently published two papers focused on the methodological aspects of protein quantitation by liquid chromatography coupled to tandem mass spectrometry, using iTRAQ labeling.

The work by Casado-Vela et al., (Proteomics, 2010) in collaboration with members of Universidad de Alicante and Universidad Pompeu Fabre, was focused on the design of new standards to improve the relative quantification of proteins by iTRAQ. The second paper, by Rodríguez-Suárez et al. (Proteomics, 2010), completed in collaboration with Dr. Matthiesen from the University of Porto and the Metabolomics and Bioinformatics Unit at CIC bioGUNE, covers some methodological and bioinformatics aspects of iTRAQ quantitation.