Course

3rd Basic Course in Proteomics

Felix Elortza- Ibon Iloro- Mikel Azkargorta, PhD

9:30-10:00 Introduction, by Felix Elortza - Proteomic analysis: General considerations. - Experiments you can do prior to mass spectrometry analysis - What can mass spectrometry offer you in proteomics (& peptidomics) - Mass spectrometry: Some basic concepts. 10:00-11:00 MALDI TOF analysis, by Ibon Iloro - Protein Identification by Peptide Mass Fingerprinting (PMF) and Peptide Fragment Fingerprinting (PFF). - How to analyze data obtained by MALDI TOF and MALDI TOF/TOF. - Molecular weight determination: Linear MALDI and ESI-TOF. - MALDI-TOF Tissue Mass Spectrometry: MALDI Imaging or New Molecular Histology. 11:00-11:30 Coffee break 11:30-12:30 nLC MS/MS analysis: Protein Identification & Characterization and Label Free Quantification by Mikel Azkargorta - Introduction to proteomic analysis by nLC MS/MS - Sample preparation for nLC MS/MS analysis. - Applications of nLC MS/MS: 1. Characterization of protein mixtures 2. Analysis of protein complexes 3. Post-translational modification (PTM) analysis 4. Differential protein expression analysis