The Fast and the Furious - What we know from NMR about the Kine:cs of Transient Encounters of Carbohydrates with Proteins

 

Seminar

The Fast and the Furious - What we know from NMR about the Kine:cs of Transient Encounters of Carbohydrates with Proteins

Thomas H. Peters

The Fast and the Furious - What we know from NMR about the Kine:cs of Transient Encounters of Carbohydrates with Proteins With few exceptions, the interactions of mono- and oligosaccharides with proteins are characterized by low affinities with dissociation constants KD in the mM range. Yet, the interactions are highly specific and biologically relevant. In many cases, nature modulates these low affinities by multivalent glycan presentation on, e.g., cell surfaces. The measurement of affinities and other thermodynamic parameters is important to understand these binding reactions. However, a complete picture also requires knowledge of the kinetic parameters such as association and dissociation rate constants kon and koff. In cells, lifetimes of glycan-protein complexes need to be regulated to stay in tune with other processes, and the main adjusting screw here is koff. In this seminar I will demonstrate that NMR offers powerful experimental approaches to explore the binding kinetics of carbohydrate-protein interactions. References: Flügge, F.; Peters, T. Insights into Allosteric Control of Human Blood Group A and B Glycosyltransferases from Dynamic NMR. ChemistryOpen 2019, 8 (6), 760-769. DOI: 10.1002/open.201900116 (acccessed 2019/07/14). Maass, T.; Ssebyatika, G.; Brückner, M.; Breckwoldt, L.; Krey, T.; Mallagaray, A.; Peters, T.; Frank, M.; Creutznacher, R. Binding of Glycans to the SARS CoV-2 Spike Protein, an Open Question: NMR Data on Binding Site Localization, Affinity, and Selectivity. Chemistry – A European Journal 2022, 28 (71), e202202614. DOI:
https://doi.org/10.1002/chem.202202614 (acccessed 2022/12/11).