CANCELLED: How HPV traffics to the nucleus (and you thought traffic in Barcelona was crazy)

2020/03/27 Atrio 800

Seminar

CANCELLED: How HPV traffics to the nucleus (and you thought traffic in Barcelona was crazy)

CANCELLED Prof. Daniel DiMaio

CANCELLED: How HPV traffics to the nucleus (and you thought traffic in Barcelona was crazy) In order for the non-enveloped human papillomavirus particle to traffic from the cell surface to the nucleus during virus entry, it needs to enter the retrograde transport pathway. After endocytosis, a short cell-penetrating peptide sequence at the C-terminus of the HPV minor capsid protein L2 drives this segment of the L2 protein through the endosome membrane into the cytoplasm. This allows retromer, a cytoplasmic cellular trafficking machine, to bind directly to the L2 protein and transfer the incoming virus particle into the retrograde pathway. Based on this mechanistic understanding of HPV entry, we have developed short soluble peptides from the L2 protein that can enter cells, titrate retromer away from the incoming virus particle, and abort infection. In addition, we have developed a new protein interference screen to identify and characterize additional cell proteins involved in HPV entry, including some that regulate retromer function, and to dissect the HPV trafficking pathway.

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