Nicanor Zalba Olaizola
Nicanor Zalba Olaizola
EARLY STAGE RESEARCHER
Precision Medicine and Metabolism Lab
Email: nzalba@cicbiogune.es
Phone: 4318 / 946 572 508
Address: Bizkaia Science and Technology Park, building 800, Derio (Bizkaia)

Protein stability (thermodynamic and kinetic) drives the biophysical properties of the polypeptide chain (protein folding) and the protein's concentration in the cellular environment (protein homeostasis). It is the result of a delicate balance between inter- and intramolecular interactions, which can be easily altered by mutations and/or upon changes in the composition of the surrounding media. In this context, NMR spectroscopy offers a plethora of suitable experiments to investigate protein stability. In our laboratory we are currently interested in the following topics:

  • Pharmacological chaperones. Rare diseases (~7000 identified to date) are an area of significant medical need affecting an estimated 350 million people worldwide, with ~95% having no currently approved drug treatment. They are often produced by inherited mutations affecting the activity of a protein and It is becoming increasily clear that, most frequently, a mutation destabilizes the protein/enzyme, ultimately affecting its intracellular homeostasis. In this context, pharmacological chaperones (small molecules which bind to the protein, restoring stability and activity without affecting its function) can be applied to many diseases. In our laboratory we are investigating new methods (NMR, biophysical and biochemical) for the discovery and characterization of pharmacological chaperones against a set of diseases: congenital eryhtropoietic porphyria, tyrosianemia.
     
  • Environmental modulation of enzyme stability. The high catalytic efficiency and the exquisite enantioselectivity of an enzyme has been employed in some industrial processes to upgrade their properties in order to make them more environmentally-friendly. However, large scale industrial implementation of biotechnological reactions is often limited by the marginal stability of the enzyme in the reactor conditions. In our laboratory we employ NMR and circular dichroism to investigate the effect of external crowding agents to improve the activity and stability of several enzymes. Specifically, we are investigating the mechanism for protein haloadaptation by a combined use of site directed mutagenesis and high-resolution NMR spectroscopy.

Protocolos de Histología

Latest Publications

Metabolic subtypes of patients with NAFLD exhibit distinctive cardiovascular risk profiles

Martinez-Arranz, I;Bruzzone, C;Noureddin, M;Gil-Redondo, R;Minchole, I;Bizkarguenaga, M;Arretxe, E;Iruarrizaga-Lejarreta, M;Fernandez-Ramos, D;Lopitz-Otsoa, F;Mayo, R;Embade, N;Newberry, E;Mittendorf, ...

HEPATOLOGY

2022-03-17

Exploration of Human Serum Lipoprotein Supramolecular Phospholipids Using Statistical Heterospectroscopy in n-Dimensions (SHY-n): Identification of Potential Cardiovascular Risk Biomarkers Related to SARS-CoV-2 Infection

Masuda, R;Lodge, S;Whiley, L;Gray, N;Lawler, N;Nitschke, P;Bong, SH;Kimhofer, T;Loo, RL;Boughton, B;Zeng, AX;Hall, D;Schaefer, H;Spraul, M;Dwivedi, G;Yeap, BB;Diercks, T;Bernardo-Seisdedos, ...

ANALYTICAL CHEMISTRY

2022-03-15

PI3K-regulated Glycine N-methyltransferase is required for the development of prostate cancer

Zabala-Letona, A;Arruabarrena-Aristorena, A;Fernandez-Ruiz, S;Viera, C;Carlevaris, O;Ercilla, A;Mendizabal, I;Martin, T;Macchia, A;Camacho, L;Pujana-Vaquerizo, M;Sanchez-Mosquera, P;Torrano, ...

ONCOGENESIS

2022-02-23

Depletion of mitochondrial methionine adenosyltransferase alpha 1 triggers mitochondrial dysfunction in alcohol-associated liver disease

Barbier-Torres, L;Murray, B;Yang, JW;Wang, JH;Matsuda, M;Robinson, A;Binek, A;Fan, W;Fernandez-Ramos, D;Lopitz-Otsoa, F;Luque-Urbano, M;Millet, O;Mavila, N;Peng, H;Ramani, K;Gottlieb, R;Sun, ...

NATURE COMMUNICATIONS

2022-01-28

J-Edited DIffusional Proton Nuclear Magnetic Resonance Spectroscopic Measurement of Glycoprotein and Supramolecular Phospholipid Biomarkers of Inflammation in Human Serum

Nitschke, P;Lodge, S;Kimhofer, T;Masuda, R;Bong, SH;Hall, D;Schafer, H;Spraul, M;Pompe, N;Diercks, T;Bernardo-Seisdedos, G;Mato, JM;Millet, O;Susic, D;Henry, A;El-Omar, EM;Holmes, E;Lindon, ...

ANALYTICAL CHEMISTRY

2022-01-05

S-adenosylmethionine inhibits la ribonucleoprotein domain family member 1 in murine liver and human liver cancer cells

Ramani, K;Robinson, AE;Berlind, J;Fan, W;Abeynayake, A;Binek, A;Barbier-Torres, L;Noureddin, M;Nissen, NN;Yildirim, Z;Erbay, E;Mato, JM;Van Eyk, JE;Lu, SC

HEPATOLOGY

2021-12-18

Uneven metabolic and lipidomic profiles in recovered COVID-19 patients as investigated by plasma NMR metabolomics

Bizkarguenaga, M;Bruzzone, C;Gil-Redondo, R;SanJuan, I;Martin-Ruiz, I;Barriales, D;Palacios, A;Pasco, ST;Gonzalez-Valle, B;Lain, A;Herrera, L;Azkarate, A;Vesga, MA;Eguizabal, C;Anguita, J;Embade, ...

NMR IN BIOMEDICINE

2021-10-27

Metabolomics and lipidomics in NAFLD: biomarkers and non-invasive diagnostic tests

Masoodi, M;Gastaldelli, A;Hyotylainen, T;Arretxe, E;Alonso, C;Gaggini, M;Brosnan, J;Anstee, QM;Millet, O;Ortiz, P;Mato, JM;Dufour, JF;Oresic, M

NATURE REVIEWS GASTROENTEROLOGY & HEPATOLOGY

2021-09-10

O-GlcNAcylated p53 in the liver modulates hepatic glucose production

Gonzalez-Rellan, MJ;Fondevila, MF;Fernandez, U;Rodriguez, A;Varela-Rey, M;Veyrat-Durebex, C;Seoane, S;Bernardo, G;Lopitz-Otsoa, F;Fernandez-Ramos, D;Bilbao, J;Iglesias, C;Novoa, E;Ameneiro, ...

NATURE COMMUNICATIONS

2021-08-20

Integrative Modeling of Plasma Metabolic and Lipoprotein Biomarkers of SARS-CoV-2 Infection in Spanish and Australian COVID-19 Patient Cohorts

Masuda, R;Lodge, S;Nitschke, P;Spraul, M;Schaefer, H;Bong, SH;Kimhofer, T;Hall, D;Loo, RL;Bizkarguenaga, M;Bruzzone, C;Gil-Redondo, R;Embade, N;Mato, JM;Holmes, E;Wist, J;Millet, O;Nicholson, ...

JOURNAL OF PROTEOME RESEARCH

2021-08-06