EM platform at CIC bioGUNE provides
two transmission electron microscopes and the auxiliary equipments required for
cryo-electron microscopy. The most sophisticated microscope is the
JEM-2200FS/CR, equipped with a 200kV field emission gun (FEG) and an OMEGA in-column energy filter. The energy filter included in the JEM-2200FS electron microscope allows the observation of thick samples, necessary for cryotomography experiments. The other microscope is the
JEM-1230, equipped with a 120kV thermionic gun. This microscope is available for in house and external users trained in
TEM.
Instrument list
1.-
JEM-2200FS/CR transmission electron microscope (JEOL, Japan), equipped with an ULTRASCAN 4000 SP (4008×4008 pixels) cooled slow-scan CCD camera (GATAN, UK).
2.-
JEM-1230 transmission electron microscope (JEOL, Japan), equipped with an ORIUS SC1000 (4008×2672 pixels) cooled slow-scan CCD camera (GATAN, UK). This microscope can be booked by trained users.
3.-
VITROBOT, automated vitrification robot (FEI).
4.- 3 cryotransfer holders used for cryo electron microscopy.
5.- 1 double tilt cryotransfer holder used for cryo electron microscopy.
6.- 1 ultra-high tilt cryotransfer holder used for cryo-electron tomography.
7.- Darkroom and
Z/I PHOTOSCAN scanner (ZEIS).
8.- High vacuum coating system (MED 020 BALTEC) for carbon evaporation and glow discharge.
9.- HP Workstations with linux for
data processing.
